大孔树脂联合Sephadex LH-20葡聚糖凝胶分离纯化芦荟大黄素

Separation and purification of Aloe-emodin by macroporous resin and Sephadex LH-20

中文关键词:  芦荟大黄素  大孔树脂  Sephadex LH-20葡聚糖凝胶  分离纯化  高效液相色谱法  含量测定

英文关键词:Aloe-emodin  macroporous resin  Sephadex LH-20 dextran gel  separation and purification  high performance liquid chromatography  determination

中文摘要:

      目的 对芦荟药材中芦荟大黄素进行分离纯化及含量测定。方法 以DM301、X5、DM130 3种大孔树脂的静态吸附率, 动态吸附率为指标, 分离芦荟大黄素, 用Sephadex LH-20葡聚糖凝胶纯化芦荟大黄素, 采用高效液相色谱法(high performance liquid chromatography, HPLC)测定芦荟大黄素的含量。结果 芦荟大黄素线性回归方程为Y=41131X?44.34(r2=0.9993), DM301大孔树脂联合Sephadex LH-20葡聚糖凝胶分离纯化芦荟大黄素效果好。结论 该方法快速、简便, 为芦荟中有效成分大黄素分离和测定提供了依据,为大黄素的纯化提供了参考。

英文摘要:

      Objective To isolate, purify and determine the content of aloe-emodin in aloe vera. Methods The static adsorption rate and dynamic adsorption rate of three macroporous resins of DM301, X5 and DM130 were used to separate aloe-emodin. Sephadex LH-20 glucan gel was used to purify aloe-emodin, and high performance liquid chromatography (HPLC) was used to determine aloe-emodin content. Results The linear regression equation of aloe-emodin was Y=41131X?44.3 (r2=0.9993). The separation and purification of aloe-emodin by DM301 macroporous resin combined with Sephadex LH-20 dextran gel was effective. Conclusion The method is rapid and simple, which provides a basis for the separation and determination of the active ingredient emodin in aloe, and a reference for purification.