小麦代谢组学技术及研究进展

Wheat Metabolomics Technology and Research Progress

中文关键词:  小麦、代谢组学、主成分分析、萌发、逆境胁迫

英文关键词:wheat  metabolomics  principal component analysis  germination  adversity stress

中文摘要:

      代谢组学是继基因组学和蛋白质组学之后新发展的一门学科，真正的反映在生物体内已经发生的生命活动，揭示生物体在特定时间、特定条件下的整体功能性状态，为生物体内在规律的研究提供信息，在植物学研究中得到广泛关注。本文主要介绍了代谢组学常用的高通量检测技术，如核磁共振、气相色谱-质谱联用、超高压液相色谱-四极杆飞行时间质谱联用等，数据分析方法，检索数据库等，对比了不同检测方法的优缺点。小麦作为世界上的主要的粮食作物之一，在代谢组学方面也有相关研究。本文重点概述了发芽小麦代谢组学研究的现状，通过分析代谢物及小麦理化性质，揭示了小麦萌发的主要能量来自糖酵解，同时增加有益活性成分，为了解在代谢水平上的谷物种子萌发的生化机制及代谢产物发生的系列变化提供了理论基础。

英文摘要:

      Metabolomics is a new development after genomics and proteomics. It truly reflects the life activities that have taken place in the organism, revealing the overall functional state of the organism at a specific time and under certain conditions. Providing information in regular research has received widespread attention in botanical research. This paper mainly introduces the high-throughput detection techniques commonly used in metabolomics, such as nuclear magnetic resonance, gas chromatography-mass spectrometry, ultra-high pressure liquid chromatography-quadrupole time-of-flight mass spectrometry, data analysis methods, retrieval databases, etc. Compare the advantages and disadvantages of different detection methods. As one of the world's major food crops, wheat has also been studied in metabolomics. This paper focuses on the current status of genomic wheat metabolomics research. By analyzing the physicochemical properties of metabolites and wheat, it is revealed that the main energy of wheat germination comes from glycolysis, and at the same time increases the beneficial active ingredients to understand the germination of grain seeds at the metabolic level. The biochemical mechanisms and series of changes in the occurrence of metabolites provide a theoretical basis.