高效液相色谱-串联质谱法同时测定漱口水中的11种磺胺类药物

Simultaneous determination of 11 sulfonamides in mouthrinse by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  高效液相色谱-串联质谱法  漱口水  磺胺类化合物

英文关键词:high performance liquid chromatography-tandem mass spectrometry  mouthrinse  sulfonamides

中文摘要:

      目的 建立一种高效液相色谱-串联质谱法(high performance liquid chromatography tandem mass spectrometry, HPLC-MS/MS)同时测定漱口水中11种磺胺类药物(磺胺嘧啶、磺胺噻唑、磺胺吡啶、磺胺甲基嘧啶、磺胺甲噻二唑、磺胺氯哒嗪、磺胺甲基异噁唑、磺胺二甲异噁唑、磺胺喹噁啉、磺胺硝苯、磺胺苯吡唑)残留的方法。方法 样品经超声提取后, 以Agilent Eclipse XDB-C18(10 cm×4.6 mm, 3.5 μm)色谱柱分离后在高效液相色谱-串联质谱法多反应监测模式下进行测定, 外标法定量。结果 11种磺胺类药物的检出限为15 μg/kg, 在3个添加水平范围内的平均回收率为80.3%~105.4%, 相对标准偏差均小于8.5%。结论 该方法简便、准确, 快速, 适用于漱口水中磺胺类药物的测定。

英文摘要:

      Objective To establish a method for simultaneous determination of 11 sulfonamides (sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethizole, sulfachloropyridazine, sulfamethoxazole, sulfisoxazole, sulfaquinoxaline, sulfanitran and sulfaphenazole) in mouthrinse by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods After ultrasonic extraction, the sample was separated by Agilent Eclipse XDB-C18 (10 cm×4.6 mm, 3.5 μm) column and then detected by HPLC-MS/MS with reaction monitoring mode, and the external standard method was used for quantification. Results The limits of detection for the 11 sulfonamides were 15 μg/kg. The mean recoveries at three spiked levels were in the range of 80.3%-105.4% with the relative standard deviations (RSDs) less than 8.5%. Conclusion The method is accurate, simple and rapid, which is suitable for the determination of sulfonamides in mouthrinse samples.