高效液相色谱法测定大豆磷脂类保健食品中磷脂酰胆碱含量

Determination of phosphatidylcholine from soybean phospholipid health products by high performance liquid chromatography

中文关键词:  大豆磷脂  磷脂酰胆碱  保健食品  高效液相色谱法

英文关键词:soybean phospholipids  phosphatidylcholine  health food  high-performance liquid chromatography method

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定大豆磷脂类保健食品中磷脂酰胆碱含量的分析方法。方法 采用高效液相色谱仪、Kromasil 100-5-SIL硅胶柱, 以流动相正己烷:异丙醇:水=0.25:4:1(V:V:V), 流速为0.5 mL/min, 检测波长为207 nm, 柱温为30 ℃, 进样量10 μL, 进行检测。结果 磷脂酰胆碱浓度为0.1~2.0 mg/mL时, 线性关系良好, 回归方程的相关系数r2=0.9998, 相对标准偏差(relative, standard deviation, RSD)为0.50%, 平均回收率为98.01%~98.73%。结论 该方法高效, 准确, 简便, 适合于检测大豆磷脂类保健食品中磷脂酰胆碱的含量。

英文摘要:

      Objective To establish a method for the determination of phosphatidylcholine from soybean phospholipid health products by high performance liquid chromatography (HPLC). Methods The sample was tested by HPLC with Kromasil 100-5-SIL silica gel column. Mobile phase was n-hexane: isopropanol: water=0.25:4:1 (V:V:V) with flow rate of 0.5 mL/min, the detection wavelength was 207 nm, column temperature was 30 ℃, and injection volume was 10 μL. Results When the concentration of phosphatidylcholine was 0.1-2.0 mg/mL, the linear relationship was good. The correlation coefficient of regression equation was r2=0.9998, the relative standard deviation (RSD) was 0.50%, and the average recoveries were 98.01%-98.73%. Conclusion This method is efficient, accurate and simple, which is suitable for detecting the content of phosphatidylcholine in soybean phospholipid health food.