高效液相色谱法测定食品中11种合成着色剂

Determination of 11 kinds of synthetic colorants in foods by high performance liquid chromatography

中文关键词:  高效液相色谱法  混合型弱阴离子交换反相固相萃取  合成着色剂

英文关键词:high performance liquid chromatography  SPE-PWA column  synthetic colorants

中文摘要:

      目的 建立一种同时测定食品中11种合成着色剂的高效液相色谱检测方法。方法 样品经提取液提取, SPE-PWA萃取柱净化。以Phenomenex C18色谱柱(250 mm×4.6 mm, 5 μm)分离, 优化流动相比例, 以0.02 mol/mL乙酸铵(A)、甲醇(B)、乙腈(C)为流动相进行梯度洗脱。采用二极管阵列检测器(photo-diode array, PDA)进行变波长分析。结果 11种人工合成着色剂18 min内完成分析。各着色剂的浓度与峰面积在0.5~50.0 mg/L浓度范围内具有良好的线性关系, 相关系数(r)在0.99989~1.00000之间。该方法的检出限为0.2 mg/kg, 定量限为0.5 mg/kg, 在0.5、2.0、10.0 mg/kg 3个添加水平的回收率为72.6%~106.3%, 相对标准偏差(relative standard deviation, RSD)小于7.8%(n=6)。结论 本方法快速、准确、灵敏、高效, 适合食品中合成着色剂的含量测定。

英文摘要:

      Objective To establish a method for the simultaneous determination of 11 kinds of synthetic colorants in foods by high performance liquid chromatography (HPLC). Methods The foods samples were extracted and purified by SPE-PWA column, separated by Phenomenex C18 column (250 mm×4.6 mm, 5 μm), and eluted by gradient of 0.02 mol/mL ammonium acetate (A), methanol (B) and Acetonitrile (C) as mobile phase. Photo-diode array (PDA) was used for variable wavelength analysis. Results This method completed the separation of 11 kinds of synthetic colorants in 18 min. The 11 kinds of synthetic colorants had good linear relationships between peak area and concentration of synthetic colorant in the range of 0.5-50.0 mg/L, and the correlation coefficients were 0.99989-1.00000. The limits of detection were 0.2 mg/kg, and the limits of quantitative were 0.5 mg/kg. The recoveries at 3 spiked levels (0.5, 2.0, 10.0 mg/kg) were 72.6%-106.3%, with the relative standard deviations less than 7.8% (n=6). Conclusion The method is rapid, accurate and sensitive, which is suitable for the determination of synthetic colorants in foods.