柱前衍生-高效液相色谱-二极管阵列法测定葡萄酒中组胺含量

Determination of histamine in wine by pre-column derivatization-high performance liquid chromatography-diode array detector

中文关键词:  柱前衍生  高效液相色谱-二极管阵列法  葡萄酒  组胺

英文关键词:pre-column derivatization  high performance liquid chromatography-diode array detector  wine  histamine

中文摘要:

      目的 建立柱前衍生-高效液相色谱-二极管阵列法(high performance liquid chromatography-diode array detector, HPLC-DAD)测定葡萄酒中组胺含量的方法。方法 将葡萄酒样品与10 mg/mL的丹磺酰氯在70 ℃柱前衍生10 min, 衍生样品以乙腈和0.01 mol/L乙酸铵溶液(含0.1%乙酸)为流动相进行等度洗脱, 于波长254 nm处进行检测。结果 该方法的标准曲线回归方程为Y=0.0319X+0.00059, 0.1~100 μg/mL的线性范围内, 组胺标准品峰面积与内标物峰面积的比值与组胺含量线性关系良好, 相关系数r2 为0.9987, 回收率为92.3%~105.2%, 检出限为0.05 μg/mL。结论 该方法操作简便、准确度高, 适用于葡萄酒中组胺含量测定。

英文摘要:

      Objective To establish a method for determination of histamine in wine by pre-column derivatization-high performance liquid chromatography-diode array detector (HPLC-DAD). Methods The wine sample was derigaved with 10 mg/mL dansyl chloride for 10 min at 70 ℃. The derived sample was detected at 254 nm after isocratic elution with acetonitrile and 0.01 mol/L ammonium acetate solution (containing 0.1% acetic acid) as the mobile phase. Results The standard curve regression equation of this method was Y=0.0319X+0.00059, with the linear range of 0.1-100 μg/mL. The ratio of the peak area of the histamine standard to the peak area of the internal standard was linearly related to the histamine content, the correlation coefficient r2 was 0.9987, the recovery was 92.3%-105.2%, and the limit of detection was 0.05 μg/mL. Conclusion This method is simple, rapid and accurate, which is suitable for the determination of histamine in wine.