高效液相色谱-蒸发光散射检测法同时测定牛舌草提取液中的5种单糖

Simultaneous determination of 5 kinds of monosaccharides in Borago officinalis L. extract by high performance liquid chromatography-evaporative light scattering detector

中文关键词:  高效液相色谱法  蒸发光散射检测器  牛舌草  单糖

英文关键词:high performance liquid chromatography  evaporative light scattering detector  Borago officinalis L.  monosaccharides

中文摘要:

      目的 建立同时测定牛舌草中5种水溶性糖(鼠李糖、阿拉伯糖、果糖、葡萄糖、半乳糖)的液相色谱-蒸发光散射分析方法。方法 各单糖组分经GRACE Prevail ES糖分析柱分离并与蒸发光散射检测器(evaporative light scattering detector, ELSD)联用, 采用V(乙腈): V(水)=72: 28作为流动相, 流速为1.0 mL/min, 蒸发光散射检测器漂移管温度为80 ℃, 空气作为载气, 流速为2.0 L/min。结果 各单糖的峰面积标准曲线在0.2000~6.400 μg范围内均具有良好的线性关系, 相关系数在0.999以上, 加样回收率在97.94%~103.3%之间; 相对标准偏差在1.15%~3.08%之间; 所建立的方法对5种糖的检测限分别为60.00、100.0、100.0、60.00、80.00 ng。结论 本方法灵敏、准确, 可用于牛舌草中水溶性糖含量的质量控制与分析。

英文摘要:

      Objective To establish a method for simultaneous determination of 5 kinds of monosaccharides (rhamnose, fructose, arabinose, glucose and galactose) in Borago officinalis L. extract by high performance liquid chromatography with evaporative light scattering detector (ELSD). Methods The monosaccharides samples were separated by GRACE Prevail carbohydrate ES column using acetonitrile-water (72:28, V:V) as the mobile phase at a flow rate of 1.0 mL/min. Drift tube temperature was 80 ℃ and air carrier gas flow rate was 2.0 L/min. Results The peak area of each monosaccharide had good linear relationships in the range of 0.2000-6.4000 μg, with the correlation coefficients all above 0.999. The recoveries were ranging from 97.94% to 103.3%, with the relative standard deviations of 1.15%-3.08%. The limits of detection for the 5 kinds of monosaccharides were 60.00, 100.0, 100.0, 60.00 and 80.00 ng, respectively. Conclusion The method is sensitive and accurate, which can be used for the quality control and analysis of water-soluble monosaccharides in Borago officinalis L.