固相萃取液相色谱-串联质谱法测定食品中6种黄曲霉毒素

Determination of 6 kinds of aflatoxins in food by solid phase extraction and liquid chromatography-tandem mass spectrometry

中文关键词:  黄曲霉毒素  固相萃取  液相色谱-串联质谱法

英文关键词:aflatoxin  solid phase extraction  liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立固相萃取-液相色谱-串联质谱法测定食品中黄曲霉毒素的含量。方法 选取19种固相萃取小柱, 在3种萃取机制下, 通过标准品的回收实验筛选合适的小柱, 然后通过样品加标实验考察小柱的净化能力。结果 在正相萃取机制下, C8固相萃取小柱符合检测结果要求, 6种黄曲霉毒素检出限均达到1.0 μg/kg, 平均加标回收率为82.2%~104.1%, 相对标准偏差为2.2%~17.8%。结论 该方法适用于食品中黄曲霉毒素的快速检测, 为相关检测机构提供参考。

英文摘要:

      Objective To establish a method for determination of aflatoxins in food by solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods A total of 19 kinds of solid-phase extraction columns were selected. Under the three extraction mechanisms, appropriate small columns were selected through the recovery experiment of standard products, and then the purification capacity of small columns was investigated through sample labeling test. Results Under the principle of positive phase extraction, the small column of C8 solid phase extraction met the requirements of the test results. The limits of detection of 6 kinds of aflatoxins were all reached 1.0 μg/kg, the average recovery rates were 82.2%-104.1%, and the relative standard deviations were 2.2%-17.8%. Conclusion This method is applicable to the rapid detection of aflatoxin in food and provides references for relevant testing institutions.