贵州地区“紫红龙”火龙果采后病原菌分离鉴定

Isolation and identification of pathogen from Hylocereus undatus cv. Zihonglong in Guizhou province

中文关键词:  “紫红龙”火龙果  采后病害  病原菌  桃吉尔霉

英文关键词:Hylocereus undatus cv. Zihonglong  postharvest disease  pathogen  Gilbertella persicaria

中文摘要:

      目的 分离鉴定贵州地区“紫红龙”火龙果在采后贮藏期间自然发病的病原菌。方法 “紫红龙”火龙果为试材, 通过涂布平板分离法以及划线纯化, 从贮藏期火龙果中分离、纯化采后病原菌, 并结合形态学观察与rDNA ITS (internal transcribed spacer)序列测定进行准确鉴定。结果 分离出1株真菌, 经鉴定该株病原菌为桃吉尔霉(Gilbertella persicaria)。结论 本研究在贵州地区腐烂的火龙果中首次分离得到G. persicaria, 该菌对红肉火龙果有较强的致病力。

英文摘要:

      Objective To isolate and identify the pathogenic bacteria of Hylocereus undatus cv. Zihonglong in Guizhou that naturally occurred during postharvest storage. Methods With Hylocereus undatus cv. Zihonglong as test material, the fungal were isolated and purified from dragon fruit during storage period by the spread plate and streak plate method, and it was accurately identified by the morphology studies and rDNA ITS (internal transcribed spacer) sequence analysis. Results One fungus was isolated, and the fungal was identified to be Gilbertella persicaria. Conclusion G. persicaria is first isolated from rotten dragon fruit in Guizhou province, and it has strong pathogenicity to red pitaya fruits.