锁阳多糖对巨噬细胞RAW264.7的免疫调节作用的影响

Effect of polysaccharide from Cynomorium songaricumon on the immunoregulatory effect of RAW264.7 macrophages cell

中文关键词:  锁阳  多糖  巨噬细胞RAW264.7  免疫调节作用

英文关键词:Cynomorium songaricum  polysaccharide  macrophage cell RAW264.7  immunomodulatory effect

中文摘要:

      目的 研究锁阳多糖对巨噬细胞RAW264.7免疫调节作用的影响。方法 以巨噬细胞RAW264.7为研究对象, 设置空白对照组、阳性(LPS)对照组、锁阳多糖给药组(25、50、100、200、400 μg/mL), 用CCK-8法测定细胞增殖活性, 中性红法测定其吞噬活性; Griess法测定其对NO分泌量的影响; ELISA法测定其IL-6和TNF-α的释放能力。结果 与空白组比较, 不同浓度(25~400 μg/mL)锁阳多糖均能够促进巨噬细胞RAW264.7的增殖(P<0.05或P<0.01), 干预24 h和48 h的增殖活性明显高于干预6 h和12 h的增殖活性(P<0.05); 锁阳多糖(25~ 400 μg/mL)能显著促进细胞的吞噬活性(P<0.05); 干预48 h后, 锁阳多糖(25~400 μg/mL)的NO分泌量显著高于空白组和LPS组(P<0.05); 与空白组和LPS组相比, 锁阳多糖(25~400 μg/mL)能显著促进细胞IL-6和TNF-α的释放(P<0.01)。结论 锁阳多糖在一定浓度范围内对巨噬细胞RAW264.7具有良好的免疫调节作用。

英文摘要:

      Objective To study the effects of polysaccharide from Cynomorium songaricum for RAW264.7 macrophages cell immune regulation. Methods RAW264.7 macrophage cell were taken as the research object and divided into the control group, positive control group (LPS), and drug experimental groups (25, 50, 100, 200, 400 μg/mL). Cell proliferation activity was determined by CCK-8 method and phagocytic activity was determined by neutral red method. The effect on NO secretion was determined by Griess method, and the release capacity of il-6 and TNF-α were measured by ELISA. Results Compared with the control group, Cynomorium songaricum polysaccharide (25-400 μg/mL) could promote the proliferation of RAW264.7 macrophages cell (P<0.05 or P<0.01), and the proliferation activity at 24 h and 48 h after intervention was significantly higher than that of at 6 h and 12 h (P<0.05). Cynomorium songaricum polysaccharide (25-400 μg/mL) could significantly promote the phagocytic activity (P<0.05). After 48 hours of intervention, the NO secretion was significantly higher than that of blank group and LPS group (P<0.05). Compared with blank group and LPS group, Cynomorium songaricum polysaccharide (25-400 μg/mL) could significantly promote the release of IL-6 and TNF-α (P<0.05 or P<0.01). Conclusion Cynomorium songaricum polysaccharide enhances the immunomodulatory effect of RAW264.7 macrophage cell in a certain concentration range.