高效液相色谱法同时测定果蔬中6种植物激素的残留

Determination of 6 kinds of plant hormones residues in fruits and vegetables by high performance liquid chromatography

中文关键词:  植物激素  高效液相色谱法  残留  果蔬

英文关键词:plant hormones  high performance liquid chromatography  residues  fruits and vegetables

中文摘要:

      目的 建立一种可同时测定水果蔬菜中的6种植物激素 (玉米素、吲哚乙酸、6-苄基腺嘌呤、脱落酸、吲哚丁酸和α-萘乙酸) 残留的高效液相色谱方法。方法 将果蔬样品中的植物激素用80%甲醇提取, 对变波长高效液相色谱法的检测条件进行优化, 优化的色谱条件为: Agilent ZORBAX Eclipse Plus C18 (150 mm×4.6 mm, 5 μm)反相色谱柱, 流动相A为甲醇, 流动相B为磷酸-氢氧化钠缓冲溶液(0.1 mol/L, pH=3.0), 流速为1.0 mL/min, 等度洗脱、变波长检测。采用该方法进行精密度和加标回收率实验。结果 对4种果蔬样品进行了精密度和加标实验, 6种植物激素的相对标准偏差(relative standard deviation, RSD)为0.21%~8.90%, 回收率为73.84%~128.40%, 最低检出限为1.4 ng/mL。结论 该方法操作简单、精确可靠、重复性好, 适合测定果蔬中6种植物激素的残留。

英文摘要:

      Objective To establish a method for simultaneous determination of 6 kinds of plant hormones (including zeatin, indole acetic acid , 6-benzyl adenine, abscisic acid, indole butyric acid and α-naphthyl acetic acid) residues in fruits and vegetables by high performance liquid chromatography (HPLC). Methods The plant hormones in fruit and vegetable samples were extracted with 80% methanol solution. The detection conditions of the HPLC method with variable wavelength detector were optimized. The reversed phase column was Agilent ZORBAX Eclipse Plus C18 (150 mm×4.6 mm, 5 μm). The mobile phase consisted of solution A was methanol and solution B was phosphate-sodium hydroxide buffer (0.1 mol/L, pH 3.0) with isocratic elution and variable wavelength detection. The flow rate was 1.0 mL/min. The recoveries of standard addition and the precision were also tested. Results Precision and recoveries experiments were conducted on 4 fruit and vegetable samples. The relative standard deviations (RSDs) of 6 kinds of plant hormones were 0.21%-8.90%, and the average recoveries 73.84%~128.40%. The limits of detection for the 6 kinds of plant hormones were 1.4 ng/mL. Conclusion This method is easy, accurate, reliable and reproducible, which is suitable for detecting the plant hormones residues in fruits and vegetables samples.