气相色谱法测定保健品及原料中角鲨烯的含量

Determination of squalene in functional food and raw materials by gas chromatography

中文关键词:  保健品  角鲨烯  气相色谱法  内标

英文关键词:functional food  squalene  gas chromatography  internal standard

中文摘要:

      目的 建立气相色谱法(gas chromatography, GC)检测保健品及原料中角鲨烯含量的分析方法。 方法 样品采用正己烷提取, 稀释至适当浓度后直接进入气相色谱仪, 采用氢火焰离子化检测器(flame ionization detector, FID)检测。采用角鲨烷为内标进行内标法定量。进样口温度280 ℃, 柱温箱温度250 ℃, 检测器温度300 ℃, 载气流速为2 mL/min, 分流比为10:1 (V:V)。结果 色谱分离过程可在10 min内完成, 角鲨烯在3~300 μg/mL 范围内线性良好, 相关系数0.9998, 检出限为1 μg/mL, 3个浓度水平的加标回收率为94.4%~101%。样品处理后在24 h内稳定性良好。结论 本方法快速、准确、可靠、前处理过程简单、分析时间短, 可用于保健品及原料中角鲨烯含量的测定。

英文摘要:

      Objective To establish a method for the determination of squalene in health products and raw materials by gas chromatography (GC). Methods The sample was extracted with n-hexane and diluted to an appropriate concentration. Then it was directly injected into the GC and detected by hydrogen flame ionization detector (FID). Squalane was used as an internal standard for quantification. The injection port temperature was 280 ℃, the column oven temperature was 250 ℃, the detector temperature was 300 ℃, the flow rate of carrier gas was 2 mL/min, and the split ratio was 10:1 (V:V). Results The chromatographic separation could be completed within 10 min. Squalene had linear relationships in the range of 3-300 μg/mL, with a correlation coefficient of 0.9998. The limit of detection was 1 μg/mL, and the recoveries at 3 concentration levels were 94.4%-101%. The stability of the sample was good within 24 h after treatment. Conclusion This method is rapid, accurate, reliable, the pretreatment process is simple and the analysis time is short, which can be used for the determination of squalene in health care products and raw materials.