超高效液相色谱法测定饮料中咖啡因含量

Determination of caffeine in beverages by ultra performance liquid chromatography

中文关键词:  超高效液相色谱法  饮料  咖啡因

英文关键词:ultra-high performance liquid chromatography  beverages  caffeine

中文摘要:

      目的 建立超高效液相色谱法测定饮料中咖啡因含量。方法 样品经预处理后, 所得溶液均过 0.22 μm滤膜, 经C18色谱柱分离, 以水和甲醇为流动相, 梯度洗脱, 流速为0.21 mL/min, 二极管陈列检测器进行分析。结果 咖啡因为1.0~100 μg/mL浓度范围内呈线性关系, 线性相关系数为0.9994, 加标回收率在90.3%~98.4 %之间, 相对标准偏差小于10 %(n=6), 检出限为0.05 mg/kg。结论 该方法操作简单、干扰小, 准确度可靠, 能够分析饮料中咖啡因。

英文摘要:

      Objective To establish a method for determination of caffeine in beverages by ultra performance liquid chromatography. Methods After pretreatment, the obtained solution was filtered through 0.22 μm membrane. The samples were separated with C18 column at the flow rate of 0.21 mL/min by gradient elution using water-methanol solution, and analyzed by diode array detector. Results The caffeine had a good linear relationship in the concentration of 1.0-100 μg/mL, and the correlation coefficients were all greater than 0.9994. The average rates of recovery were 90.3%-98.4%, and the relative standard deviations were less than 10%（n=6）. The limit of detection of caffeine was 0.05 mg/kg. Conclution This method is simple, small interference, high accuracy, which is suitable for determination of caffeine in beverages.