分光光度法测定宁神颗粒中总皂苷含量

Determination of total saponins in Ningshen granules by spectrophotometry

中文关键词:  总皂苷  宁神颗粒  分光光度法

英文关键词:total saponins  Ningshen granules  spectrophotometry

中文摘要:

      目的 建立分光光度法测定宁神颗粒中总皂苷含量。方法 采用Amberlite-XAD-2大孔树脂层析柱富集法纯化总皂苷, 以香草醛－高氯酸溶液为显色剂使显色, 采用分光光度法(λ＝560 nm)对宁神颗粒中总皂苷的含量进行测定。结果 宁神颗粒中总皂苷浓度在31.76~190.6 μg/mL范围内呈良好线性关系(r=0.9996); 低、中、高3个浓度的平均回收率分别为97.7%、98.9%和99.2%, 相对标准偏差(relative standard deviation, RSD)小于2.2%。本方法的检出限为3.4 μg/g, 定量限为11.2 μg/g。结论 本方法简便、准确、成本低, 可用于宁神颗粒中总皂苷的含量测定, 对产品进行质量控制。

英文摘要:

      Objective To establish a method for the determination of total saponins in the Ningshen granules by spectrophotometry. Methods Total saponins was purified by Amberlite-XAD-2 macroporous resin column enrichment method, color development with vanillin-perchloric acid solution as color developer. The content of total saponins in Ningshen granules was determined by spectrophotometry (λ=560 nm). Results The linear range of total saponins in Ningshen granules was 31.76-190.6 μg/mL(r=0.9996). The average recoveries of low, medium and high concentrations were 97.7%, 98.9% and 99.2%, respectively. The relative standard deviation (RSD) was less than 2.2%. The limit of detection was 3.4 μg/g and the limit of quantitation was 11.2 μg/g. Conclusion This method is simple, accurate and has low cost, which can be used for determining the content of total saponins in Ningshen granules and controlling the quality of products.