高效液相色谱法同时测定保健食品软胶囊剂型中五味子甲素、五味子乙素和五味子醇甲

Simultaneous determination of schisandrin, schizandrin A and schizandrin B in health food soft capsule dosage form by high performance liquid chromatography

中文关键词:  高效液相色谱法  保健食品  软胶囊  五味子甲素  五味子乙素  五味子醇甲

英文关键词:high performance liquid chromatography  health food  soft capsule  schizandrin A  schizandrin B  schisandrin

中文摘要:

      目的 优化样品前处理条件, 建立高效液相色谱法(high performance liquid chromatography, HPLC)同时测定保健食品软胶囊剂型中五味子甲素、五味子乙素和五味子醇甲3种成分含量的方法。方法 样品经环己烷溶解后, 经过甲醇萃取, 被测成分采用CAPCELL PAK-C18(4.6 mml.D×250 mm, 5 μm)色谱柱分离, 以甲醇-水作为流动相进行梯度洗脱, 流速为1.0 mL/min。结果 保健食品软胶囊剂型中五味子甲素、五味子乙素和五味子醇甲3种成分的线性范围分别为0~295、0~303、0~5022 μg/mL, 相关系数为0.9992~0.9995, 线性良好; 对五味子甲素、五味子乙素和五味子醇甲的进行高、中、低3种水平浓度下进行加标回收实验, 回收率为89.1%~97.8%; 相对标准偏差(relative standard deviation, RSD)≤3.9%(n=6)。结论 该方法前处理简单, 灵敏度及加标回收率高, 准确度和稳定性好, 可以满足保健食品软胶囊剂型中五味子甲素、五味子乙素和五味子醇甲的分离和含量测定。

英文摘要:

      Objective To optimize the sample pretreatment conditions and establish a method for simultaneous determination of schisandrin, schizandrin A and schizandrin B in health food soft capsule by high performance liquid chromatography (HPLC). Methods After the sample was dissolved in cyclohexane and extracted with methanol, the measured components were separated by a CAPCELL PAK-C18 chromatographic column (4.6 mml.D × 250 mm, 5 μm), and the gradient elution was carried out with methanol-water as the mobile phase at a flow rate of 1.0 mL/min. Results The 3 components of health food soft capsule had good linear correlation coefficient of 0.9992-0.9995, and the linear range was 0-295, 0-303, 0-5022 μg/mL, respectively. The recovery rate of schisandrin, schizandrin A and schizandrin B was 89.1%-97.8%, respectively. Relative standard deviation (RSD) was less than 3.9% (n=6). Conclusion The method has simple pre-treatment, high sensitivity and standard recovery, good accuracy and stability, which is suitable for simultaneous determination of schisandrin, schizandrin A and schizandrin B in health food soft capsule.