高效液相色谱法同时检测黄油及无水奶油中苯甲酸、山梨酸、安赛蜜、糖精钠

Simultaneous determination of benzoic acid, sorbic acid, acesulfame and saccharin sodium in butter and anhydrous butter by high pressure liquid chromatography

中文关键词:  苯甲酸  山梨酸  安赛蜜  糖精钠  高效液相色谱法  同时检测

英文关键词:benzoic acid  sorbic acid  acesulfame  saccharin sodium  high pressure liquid chromatography  simultaneous detection

中文摘要:

      目的 建立黄油及无水奶油中2种甜味剂(安赛蜜、糖精钠)及2种防腐剂(苯甲酸、山梨酸)含量的高效液相色谱分析方法。方法 样品去除脂肪后, 用甲醇-水(1:1, V:V)提取, 提取液使用C8色谱柱 (250 mm× 4.6 mm, 5 μm)、在波长为230 nm、流动相为1.0 mL/min的条件下进行检测。结果 苯甲酸、山梨酸、安赛蜜、糖精钠在0~100 μg/mL范围内, 浓度与峰面积有良好的线性关系, 相关系数大于0.99, 检出限分别为0.87、0.91、2.75、2.91 mg/kg; 样品的加标平均回收率分别为93.12%~102.34%、93.02%~103.23%、91.53%~108.16%、93.48%~105.46%, 相对标准偏差均小于10%。结论 本方法可适用于黄油及无水奶油中苯甲酸、山梨酸、安赛蜜、糖精钠含量同时测定。

英文摘要:

      Objective To establish a method for the determination of 2 sweeteners (acesulfame and saccharin sodium) and 2 preservatives (benzoic acid and sorbic acid) in butter and anhydrous cream by high performance liquid chromatography (HPLC). Methods After the fat was removed from the sample, the mixture of methanol and water (1:1=V:V) was used as an extracting agent. The extracting solution was analyzed in C8 chromatographic column by HPLC under the conditions of 230 nm in wavelength and 1.0 mL/min in the speed of mobile phrase. Results Benzoic acid, sorbic acid, acesulfame and saccharin sodium had good linear relationships in the range of 0-100 μg/kg and the correlation coefficients were larger than 0.99. The limits of detection were 0.87, 0.91, 2.75, 2.91 mg/kg, respectively. The average standard recoveries of samples were 93.12%-102.34%, 93.02%-103.23%, 91.53%-108.16%, 93.48%-105.46%, respectively. The relative standard deviations were less than 10%. Conclusion This method can be used for simultaneous determination of benzoic acid, sorbic acid, acesulfame and saccharin sodium in butter and anhydrous butter.