敌百虫分子印迹聚合物的制备及应用

Preparation and application of molecularly imprinted polymers of trichlorfon

中文关键词:  分子印迹  敌百虫  固相萃取

英文关键词:molecular imprinting  trichlorfon  solid phase extraction

中文摘要:

      目的 制备分子印迹聚合物, 为敌百虫的分离富集提供参考。方法 以敌百虫为模板, 3-氨基三丙基乙氧基硅烷(3-aminotripropylethoxysilane, APTES)为功能单体, 正硅酸乙酯(ethyl orthosilicate, TEOS)为交联剂, 采用溶胶-凝胶法制备分子印迹聚合物。将制备的印迹聚合物作为固相萃取填料应用于实际样品中敌百虫的分离富集, 结合高效液相色谱法(high performance liquid chromatography, HPLC)对白菜、甘蓝、西红柿中的敌百虫含量进行测定。结果 该方法可有效去除基质干扰, 检出限为0.92 μg/kg, 回收率在87.66%~100.0%之间, RSD≤4.5%。结论 本实验为实际样品的前处理提供了新思路, 为敌百虫的检测发展了新方法, 有望广泛应用于实际样品中敌百虫的分离检测中。

英文摘要:

      Objective To prepare the molecularly imprinted polymers and provide a reference for the separation and enrichment of trichlorfon. Methods Molecularly imprinted polymer was prepared using sol-gel method, with trichlorfon as template, 3-aminotripropylethoxysilane (APTES) as functional monomer, and ethylorthosilicate (TEOS) as crosslinking agent. The prepared imprinted polymer was used as the solid phase extraction filler for the separation and enrichment of trichlorfon in the actual samples. In combination with high performance liquid chromatography (HPLC), the content of trichlorfon in Chinese cabbage, cabbage, and tomato was determined. Results The results showed that the matrix interference could be effectively removed in this method. The limit of detection was 0.92 μg/kg, and the recovery rates were between 87.66% to 100.0%, with RSD of not greater than 4.5%. Conclusion This experiment provides a new idea for the pretreatment of actual samples and develops a new method for the detection of trichlorfon. It is expected to be widely used in the separation and detection of trichlorfon in actual samples.