环介导等温扩增法快速检测食源性致病菌

Rapid detection of foodborne pathogenic bacteria by loop-mediated isothermal amplification

中文关键词:  恒温扩增  PCR  致病菌  检测  快速

英文关键词:thermostatic amplification  PCR  pathogenic bacteria  detection  rapid

中文摘要:

      目的 考察环介导等温扩增技术在食源性致病菌快速检测中的应用效果。方法 待检样品按照GB 4789系列标准进行前增菌后, 提取核酸进行致病菌检测。结果 食品中6种常见的致病菌: 金黄色葡萄球菌、沙门氏菌、志贺氏菌、单增李斯特菌、副溶血弧菌和大肠杆菌O157检测阳性符合率100%, 阴性符合率100%, 检出限介于3.0?103~1.1?104 CFU/25 g, 前增菌时间最快5 h, 检测时间为2 d。结论 环介导等温扩增技术具有检出限低、特异性好、检测速度快等优点, 能较好地满足进出口食品中致病菌快速检测的要求。

英文摘要:

      Objective To investigate the application of loop-mediated isothermal amplification (LAMP) in the rapid detection of foodborne pathogenic bacteria. Methods The samples were pre-enriched according to the GB 4789 series standard and nucleic acids were extracted for the detection of pathogens. Results The six common pathogens in food: Staphylococcus aureus, Salmonella, Shigella, Listeria monocytogenes, Vibrio parahaemolyticus and E. coli O157 were tested, and both the positive coincidence rates and the negative coincidence rates were 100%. The limit of detection was between 3.0×103-1.1×104 CFU/25 g. The shortest pre-enriched time was 5 hours and the total detection time was 2 d. Conclusion The loop-mediated isothermal amplification has the advantages of low detection limit, good specificity, and high detection speed, which can better meet the requirements for rapid detection of pathogenic bacteria in imported and exported foods.