超高效液相色谱-三重四级杆质谱法同时检测葡萄酒中16种高关注度化合物

Simultaneous determination of 16 kinds of high-concerned compounds in wine by ultra performance liquid chromatography-tandem mass spectroscopy

中文关键词:  高效液相色谱-串三重四级杆质谱法  葡萄酒  高关注度化合物  风险因子  同时检测

英文关键词:ultra performance liquid chromatography-tandem mass spectroscopy  wine  high-concerned compounds  risk factors  simultaneous determination

中文摘要:

      目的 建立一种同时快速、准确检测葡萄酒中多种高关注度风险因子的超高效液相色谱-三重四级杆质谱(ultra performance liquid chromatography-tandem mass spectroscopy, UPLC-MS/MS)的分析方法. 方法 葡萄酒样品经水分散后用超声辅助稀释, 离心后经0.22 μm滤膜过滤, 最后使用高效液相色谱-串联四级杆质谱仪进行定性和外标法定量。结果 16种高风险因子在0.001~0.020 mg/L浓度范围内呈现良好线性关系, r2大于0.995: 在0.02、0.05、0.15 mg/kg 3个添加浓度上回收率为80.1%~107.8%, 相对标准偏差(relative standard deviation, RSD)小于5.4%, 定量限在0.007~4.560 μg/kg, 检出限为0.002~1.303 μg/kg。结论 该方法操作简单, 快速可靠, 重复性好, 可满足对葡萄酒中16种高关注度风险因子检测的要求, 非常适用于政府监管部门及第三方检测机构进行葡萄酒的食品安全风险筛查。

英文摘要:

      Objective To develop a method for simultaneous determination of 16 kinds of high-concerned compounds in wine by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Wine was dispersed by water and diluted by ultra-sonic bath, then centrifuged and filtrated by 0.22 μm filter membrane. Then the samples were detected by UPLC-MS/MS and quantified by external standard method. Results The method showed a good linearity in the range of 0.001-0.020 mg/L for 16 compounds and the r2 were more than 0.995. The recoveries were in the range of 80.1%-107.8% at the 3 spiked levels of 0.02, 0.05, 0.15 mg/kg for 16 compounds, and the relative standard deviations (RSDs) were less than 5.4%. The limits of quantization were in the range of 0.007-4.560 μg/kg, and the limits of detection were 0.002-1.303 μg/kg. Conclusion This method is simple, fast and reliable, with good repeatability, which can meet the detection requirements of these 16 kinds of high-concerned risk factors in wine. It is suitable for quality control and food safety risk control in government supervision and third party inspection agency.