米仁沙BbBb牙库甫,祖丽胡玛尔BbBb阿卜杜合力力,木尼萨·迪力夏提,丛媛媛,帕丽达·阿不力孜.阿里红多糖组分对小鼠脾淋巴细胞免疫功能的影响[J].食品安全质量检测学报,2018,9(16):4369-4374
阿里红多糖组分对小鼠脾淋巴细胞免疫功能的影响
Effects of polysaccharide from Fomes officinals Ames on immune function of murine splenic lymphocytes
投稿时间:2018-05-14  修订日期:2018-08-16
DOI:
中文关键词:  阿里红多糖组分  淋巴细胞  免疫功能
英文关键词:polysaccharide from Fomes officinals Ames  lymphocytes  immunologic function
基金项目:国家自然科学基金项目(81460635)
作者单位
米仁沙BbBb牙库甫 新疆医科大学 
祖丽胡玛尔BbBb阿卜杜合力力 新疆医科大学 
木尼萨·迪力夏提 新疆医科大学 
丛媛媛 新疆医科大学 
帕丽达·阿不力孜 新疆医科大学 
AuthorInstitution
mirenshayakupu Xinjiang Medical University 
zulihumaerabuduhelili Xinjiang Medical University 
MUNISA Dilixiati Xinjiang Medical University 
CONG Yuan-Yuan Xinjiang Medical University 
PALIDA Abulizi Xinjiang Medical University 
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中文摘要:
      目的 研究阿里红多糖组分FOPS-a、FOPS-b对小鼠脾脏淋巴细胞免疫调节功能的影响。方法 实验方法选用CCK-8(Cell Counting Kit-8)试剂检测不同质量浓度阿里红多糖组分对小鼠脾脏淋巴细胞增殖率的影响; Griess法测定阿里红多糖组分对NO的释放量的影响; ELISA试剂盒测定阿里红多糖组分对IL-2、IL-4、INF-γ和IGg释放能力的影响。结果 一定质量浓度的阿里红多糖组分能促进淋巴细胞的增殖活性, 刺激淋巴细胞释放NO, 并促进淋巴细胞分泌IL-2、IL-4、INF-γ和IGg。结论 阿里红多糖组分在体外具有一定的免疫活性, 能增强小鼠脾脏淋巴细胞免疫功能。
英文摘要:
      Objective To study the effects of polysaccharide components FOPS-a and FOPS-b from Fomes officinals Ames on immunomodulatory function of spleen lymphocytes in mice. Methods The effects of different mass concentrations of Fomes officinals Ames polysaccharide components on proliferation rate of spleen lymphocytes in mice were detected by Cell Counting kit-8 reagent, the effect on the release of NO was determined by Griess method, and the effects on the release capacity of IL-2, IL-4, INF-γ and IGg were determined by the ELISA kit. Results The polysaccharide components of Fomes officinals Ames with a certain mass concentration could promote the proliferation activity of lymphocytes, stimulate lymphocytes to release NO and promote lymphocytes to secrete IL-2, IL-4, INF-γ and IGg. Conclusion Fomes officinals Ames polysaccharide components have certain immune activity in vitro and can enhance the immune function of spleen lymphocytes in mice.
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