高效液相色谱法测定面包、饼干等烘焙类食品中的脱氢乙酸

Quantitative determination of dehydroacetic acid in bread, cookies and other baked food by high performance liquid chromatography

中文关键词:  脱氢乙酸  高效液相色谱法  食品  面包

英文关键词:dehydroacetic acid  high performance liquid chromatography  food  bread

中文摘要:

      目的 建立面包、饼干等烘焙类食品中的脱氢乙酸的前处理及高效液相色谱分析方法, 并对30件该类食品样品中脱氢乙酸的含量进行测定。方法 用溶剂萃取法提取脱氢乙酸, 反相高效液相色谱法进行检测, 流动相为甲醇:0.02 mol/L乙酸铵(氨水调节pH到9.00)=5:95(V:V)。结果 本方法建立的标准曲线在1.00~ 150 μg/mL范围内具有良好的线性关系, 15.0、40.0和120 μg/mL 3个浓度的回收率范围为85.9%~108.3%, 相对标准偏差不高于7.6%。该方法的定量限为0.003 mg/g, 随机抽测的食品中的脱氢乙酸含量均未超过国家标准规定限量。结论 本方法简单、准确度高, 适合于检测面包、饼干等烘焙类食品中的脱氢乙酸。

英文摘要:

      Objective To establish a method for the pretreatment and high performance liquid chromatography analysis of dehydroacetic acid in bread, biscuits and other baked food, and determine the content of dehydroacetic acid in 30 samples of this kind of food. Methods Dehydroacetic acid was extracted by solvent extraction, and samples were determined by reversed-phase high performance liquid chromatography. The mobile phase was methanol: 0.02 mol/L ammonium acetate ( pH was adjusted to 9.00 by ammonia)=5:95(V:V). Results The standard curve established by this method had a good linear relationship in the range of 1.00-150 μg/mL. The recoveries of the 3 spiked levels of 15.0, 40.0 and 120 μg/mL were 85.9%-108.3%, and the relative standard deviations were not higher than 7.6%. The limit of quantification (LOQ) was 0.003 mg/g. The content of dehydroacetic acid in the food of random sampling did not exceed the national standard limit. Conclusion The method is simple and accurate, which is suitable for determining dehydroacetic acid in bread, biscuits and other baked food.