基于平行反应监测模式的液相色谱-四极杆-静电场轨道阱质谱法快速测定猪肉中β-受体激动剂残留

Quantitative determination of β-agonists residues in pork by liquid chromatography quadrupole-orbitrap-high resolution mass spectrometry based on parallel reaction monitoring mode

中文关键词:  β-受体激动剂  猪肉  残留  液相色谱-高分辨质谱法  平行反应监测

英文关键词:β-agonists  pork  residue  liquid chromatography-quadrupole-orbitrap mass spectrometry  parallel reaction monitoring

中文摘要:

      目的 建立一种测定猪肉中β-受体激动剂残留的液相色谱-四极杆-静电场轨道阱质谱方法。 方法 样品经乙腈提取后, 经Captiva ND lipids净化柱去除基质中的脂质干扰物, 液相色谱分离, 四极杆-静电场轨道阱质谱定性定量测定, 平行反应监测模式检测, 内标法定量测定。结果 8种待测组分均获得足够的色谱保留和分离, 各化合物在0.5~5.0 μg/kg范围内呈现良好的线性关系, 日内和日间精密度小于15%, 方法回收率在85%~115%之间, 方法的定量限为0.5 μg/kg。结论 此方法快速、准确且灵敏度高, 为监测猪肉中的β-受体激动剂残留提供了快速准确的技术手段。

英文摘要:

      Objective To establish a quantitative method for the determination of β-agonists residues in pork by liquid chromatography-quadrupole-Orbitrap mass spectrometry. Methods Samples were exacted with acetonitrile. Then the supernatant was purified by Captiva ND lipids solid phase extraction. The analytes were determined by parallel reaction monitoring mode, and quantified by internal standard. Results The matrix-matched calibration curve showed a good linear within the concentration range of 0.5-5.0 μg/kg. The intra- and inter-day precisions were less than 15%. The average recoveries of β-agonists ranged from 85% to 115%, and the limit of quantitative was 0.5 ?g/kg. Conclusion The method was rapid and highly sensitive, which provides an accurate and effective solution of detection of β-agonists in pork.