QuEChERS-气相色谱法测定马铃薯中 α-萘乙酸甲酯

Determination of α-naphthaleneacetic acid methyl ester in potatoes by QuEChERS with gas chromatography

中文关键词:  α-萘乙酸甲酯  马铃薯  气相色谱法  QuEChERS法

英文关键词:α-naphthaleneacetic acid methyl ester  potato  gas chromatography  QuEChERS method

中文摘要:

      目的 建立QuEChERS-气相色谱法(gas chromatography, GC)测定马铃薯中α-萘乙酸甲酯含量的方法。方法 样品经0.2%酸性乙腈和QuEChERS盐包提取, 旋蒸浓缩后用甲醇溶液复溶, 经QuEChERS分散固相萃取试剂盒净化, 采用毛细管柱DB-5 (30 m×0.25 mm, 0.25 μm)分离, 火焰离子化检测器检测, 外标法定量。结果 在1~100 mg/L浓度范围内, α-萘乙酸甲酯具有良好的线性关系(r=0.9998), 在0.5、2.5、5.0 mg/kg 3个浓度水平下, 回收率范围为91.2%~96.7%, 此方法的相对标准偏差(relative standard deviation, RSD)为0.75%~1.12%, 方法检出限为0.05 mg/kg, 定量限为0.2 mg/kg。结论 此方法简便、快速、准确, 可以用于马铃薯中α-萘乙酸甲酯含量的检测。

英文摘要:

      Objective To establish a method for determination of α-naphthaleneacetic acid methyl ester in potatoes by QuEChERS with gas chromatography (GC). Methods Samples were extracted by 0.2% acid acetonitrile and QuECHERS salt pack, concentrated by vortex evaporation and reconstituted by methanol, purified by QuEChERS dispersive solid phase extraction kit, separated by capillary column DB-5 (30 m×0.25 mm, 0.25 μm), and then detected by flame ionization detector and quantified by external standard method. Results α-naphthaleneacetic acid methyl ester showed a good linearity in the range of 1-100 mg/L (r=0.9998). The average recoveries were 91.2%-96.7% at the spiked levels of 0.5, 2.5, and 5.0 mg/kg with the relative standard deviations (RSDs) of 0.75%-1.12%. The limit of detection was 0.05 mg/kg and the limit of quantitative was 0.2 mg/kg. Conclusion This method is simple, rapid and accurate, which is suitable for determination of α-naphthaleneacetic acid methyl ester in potatoes.