| 罗志辉,陈丽娜,陈冠丹,韦庆敏,李娜.新型紫外可见光法测定沙田柚中维生素C的含量[J].食品安全质量检测学报,2018,9(10):2321-2325 |
| 新型紫外可见光法测定沙田柚中维生素C的含量 |
| A novel UV-visible method for the detection of vitamin C in Shatian pumelo |
| 投稿时间：2018-04-02 修订日期：2018-05-04 |
| DOI： |
| 中文关键词: 紫外可见光,开关,沙田柚,维生素C,检测 |
| 英文关键词:UV-visible turn on Shatian pumelo vitamin C |
| 基金项目:国家自然科学基金项目(21565028, 21765022)、广西高校中青年教师基础能力提升项目(2017KY0543)、广西农产品深加工与安全技术创新团队资助 |
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| Author | Institution |
| LUO Zhi-Hui | Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi,College of Chemistry and Food Science,Yulin Normal University,Yulin |
| CHEN Li-Na | Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi,College of Chemistry and Food Science,Yulin Normal University,Yulin |
| CHEN Guan-Dan | Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi,College of Chemistry and Food Science,Yulin Normal University,Yulin |
| WEI Qing-Min | Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi,College of Chemistry and Food Science,Yulin Normal University,Yulin |
| LI Na | Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi,College of Chemistry and Food Science,Yulin Normal University,Yulin |
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| 中文摘要: |
| 目的 建立一种测定沙田柚中维生素C含量的新方法。方法 采用紫外turn on法在510 nm处测定维生素C含量。优化氯化铁和邻菲啰啉的浓度、反应时间等因素。结果 获得氯化铁最佳浓度是0.01 mol/L, 反应时间为1 h。在最佳条件下, 获得校准曲线方程Y=2971X+0.0218(r=0.9997), 灵敏度为0.1 μg/mL, 并用于测定桂东南的沙田柚中的维生素C, 结果显示沙田柚中维生素C的含量为78.58 mg/100 g, 该结果与文献报道一致。结论 该方法快速、准确、灵敏, 适合水果、蔬菜的维生素C含量的测定。 |
| 英文摘要: |
| Objective To develop a novel UV-Visible method for determination of vitamin C in Shatian pumelo. Methods Vitamin C content was measured at 510 nm by UV turn on method. The concentration and reaction time of ferric chloride and ortho-phenanthroline were optimized. Results The optimum concentrations of ferric chloride and adjacent phenanthroline were 0.01 and 0.005 mol/L, respectively, and the reaction time was 1 h. Under the best conditions, the equation was obtained which was Y=2971X+0.0218(r=0.9997). The sensitivity was 0.1 μg/mL. The method was applied to detect vitamin C in Shatian pumelo in southeast of Guangxi, and the result was 78.58 mg/100 g, which was consistent with the literature. Conclusion The proposed method is rapid, accurate and sensitive, which is suitable for detecting vitamin C content of fruits and vegetables. |
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