辣椒粉中非法添加苏丹红色素快速检验分析

Rapid test and analysis of Sudan red illegally added into Capsicum annuum L.

中文关键词:  辣椒  苏丹红  非法添加  快速检验分析

英文关键词:Capsicum annuum L  Sudan red  illegally added  rapid test and analysis

中文摘要:

      目的 建立辣椒粉中苏丹红色素快速检验分析方法。方法 以苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ为对照, 以正己烷-乙酸乙酯(7:1, V:V)为展开剂, 采用薄层色谱法进行定性鉴别; 以苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ为对照, Agilent C18(250 mm×4.6 mm, 5 μm)为色谱柱, 乙睛-0.2磷酸(92:8, V:V)为流动相, 流速为1.0 mL/min, 检测波长为495 nm, 柱温30 ℃, 采用高效液相色谱法进行检测。结果 建立薄层色谱法能很好分离各成分; 苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ的线性范围分别在16.49~245.59 μg (r=0.9999)、16.30~334.88 μg(r=0.9997)、16.81~478.02 μg(r=0.9999)、16.82~438.21 μg(r=0.9996); 平均回收率(n=6)分别为97.68%、98.35%、98.32%、98.14%。结论 本法简便可行、重复性好, 可用于辣椒粉中非法添加苏丹红色素的质量控制和快速检验。

英文摘要:

      Objective To establish a method for rapid detection and analysis of Sudan red in Capsicum annuum L. Methods Sudan red Ⅰ, Sudan red Ⅱ, Sudan red Ⅲ and Sudan red Ⅳ were used as control, hexane-ethyl acetate (7:1, V:V) was used as developing agent, and thin layer chromatography (TLC) was used for qualitative identification. Sudan red Ⅰ, Sudan red Ⅱ, Sudan red Ⅲ and Sudan red Ⅳ were used for control, the chromatographic column was Agilent C18 (250 mm×4.6 mm, 5 m), mobile phase was acetonitrile-0.2 phosphate (92:8, V:V), flow rate was 1.0 mL/min, detection wavelength was 495 nm, and column temperature was 30 ℃. Samples were detected by high performance liquid chromatography (HPLC). Results The established TLC method had a good separation effect on each component. The linear ranges of Sudan red Ⅰ, Sudan red Ⅱ, Sudan red Ⅲ and Sudan red Ⅳ were 16.49~245.59 μg (r=0.9999), 16.30~334.88 μg (r=0.9997), 16.81~478.02 μg (r=0.9999) and 16.82~438.21 μg (r=0.9996), respectively. The average recovery rates (n=6) were 97.68%, 98.35%, 98.32% and 98.14%, respectively. Conclusion This method is simple, feasible and reproducible, which can be used for quality control and rapid test of Sudan red pigments added illegally in Capsicum annuum L.