高效液相色谱法测定山银花提取物中7种有机酸的含量

Simultaneous determination of 7 kinds of organic acids in Lonicerae Flos extract by high performance liquid chromatography

中文关键词:  山银花提取物  有机酸  高效液相色谱法

英文关键词:Lonicerae Flos extract  organic acids  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法同时测定山银花提取物中7种有机酸的含量。方法 准确称取一定量过筛的山银花提取物粉末经50%甲醇溶解并超声提取后, 采用Acclaim 120 C18色谱柱(4.6 mm×250 mm, 5 μm), 流动相为乙腈-0.4%磷酸溶液进行梯度洗脱, 流速为1.0 mL/min, 柱温为25 ℃, 检测波长为327 nm, 测定7种有机酸的含量。结果 7种有机酸在相应浓度区间线性良好(r>0.999), 回收率为86.3%~97.7%, 相对标准偏差<5% (n=6)。结论 该方法操作简便、结果准确、灵敏度高, 适用于山银花提取物中7种有机酸含量的测定。

英文摘要:

      Objective To establish a method for the determination of 7 kinds of organic acids in Lonicerae Flos extract by high performance liquid chromatography. Methods A certain amount of samples were weighed accurately, and dissolved with 50% methanol and extracted with ultrasounds. The samples were separated with a Acclaim 120 C18 column (4.6 mm×250 mm, 5 μm) at the flow rate of 1.0 mL/min by gradient elution using acetonitril and 0.4% phosphoric acid solution as the mobile phase. The temperature of the column oven was maintained at 25 ℃, the detection wavelength was 327 nm, and then the content of 7 kinds of organic acids was detected. Results The 7 kinds of organic acids showed a good linear correlation in certain concentration range (r>0.999), respectively. The average recoveries of 7 kinds of organic acids were 86.3%-97.7%, and the relative standard deviations were less than 5% (n=6). Conclusion This method is simple, accurate and highly sensitive, which is suitable for the determination of 7 kinds of organic acids in Lonicerae Flos extract.