气相色谱-质谱联用法检测动物源性食品中的敌鼠

Determination of diphacinone in animal-origin food by gas chromatography-mass spectrometry

中文关键词:  气相色谱-质谱联用法  敌鼠  动物源性食品

英文关键词:gas chromatography-mass spectrometry  diphacinone  animal-origin food

中文摘要:

      目的 建立气相色谱-质谱联用法(gas chromatography-mass spectrometry, GC-MS)测定动物源性食品中敌鼠的方法。方法 本研究考察了提取溶剂、萃取参数及净化小柱对检测结果的影响, 样品用丙酮-正己烷(1:1, V:V)为提取溶剂进行微波萃取, 采用C18小柱进行净化, 以GC-MS进行检测。结果 加标水平为0.001、0.002、0.01 mg/kg时, 平均回收率为62%~90%, 相对标准偏差为0.98%~9.04%, 敌鼠的方法检出限为 0.0008 mg/kg. 结论 该方法快速、简单, 且灵敏度高, 适用于动物源性食品中的敌鼠的检测。

英文摘要:

      Objective To establish a method for determination of diphacinone in animal-origin food by gas chromatography-mass spectrometry (GC-MS). Methods The effects of extraction solvents, extraction parameters and purification column on the detection results were investigated. Samples were extracted with acetone n-hexane (1:1, V:V) as solvent and microwave-assisted extraction. Then they were purified by C18 column, and detected by GC-MS. Results At spiked levels of 0.001, 0.002 and 0.01 mg/kg, the average recoveries were in the range of 62%-90%, and relative standard deviations were 0.98%-9.04%. The limit of quantification was 0.0008 mg/kg. Conclusion The method is rapid, simple and highly sensitive, which is suitable for detection of diphacinone in animal-origin food.