菲律宾蛤仔血管紧张素转化酶抑制肽大孔 树脂脱腥工艺研究

Deodorization process of Ruditapes philippinarum angiotensin I-converting enzyme inhibitory peptides with macroporous resin

中文关键词:  菲律宾蛤仔ACE 抑制肽  大孔树脂  脱腥技术

英文关键词:Ruditapesphilippinarum ACE inhibitory peptide  macroporous resin  deodorization technology

中文摘要:

      目的 确定菲律宾蛤仔血管紧张素转化酶(angiotensin I-converting enzyme, ACE)抑制肽最佳脱腥工艺。方法 采用大孔树脂脱腥法, 选取7 种大孔树脂, 以感官评定作为指标, 利用静态吸附和动态吸附2 种方法分别对菲律宾蛤仔酶解液进行脱腥效果的研究, 从中选取最佳脱腥工艺, 并对脱腥前后酶解液ACE 抑制率进行测定。结果 最佳脱腥工艺为: AB-8 型号大孔树脂, 静态吸附, 大孔树脂:酶解液=1:10(V:V), 35 ℃, 吸附脱腥时间2 h; 动态吸附, 大孔树脂:酶解液=1:10(V:V), 流速3 mL/min。在该优化条件下, 静态吸附和动态吸附的抑制率分别为46.09%、52.1%。结论 此工艺可为大规模工业化生产及产品开发提供技术参考。

英文摘要:

      Objective To determine the best deodorization process of Ruditapes philippinarum angiotensin I-converting enzyme inhibitory peptides (ACEIPs). Methods Seven kinds of macroporous resin were selected by using macroporous resin. Based on sensory evaluation, two methods including static adsorption and dynamic adsorption were used to investigate the effects of the removal of the hydrolysis of Ruditapes philippinarum. And the optimal removal process was selected and the ACE inhibition rate was determined. Results The best removal process were as follow: AB-8 macroporous resin, static adsorption, macroporous resin: enzymatic hydrolysate(volume ratio)=1:10(V:V), temperature of 35 ℃, and adsorption deodorization time of 2 h; dynamic adsorption,macroporous resin: enzymatic hydrolysate=1:10(V:V), and the flow rate of 3 mL/min. Under the optimized conditions, the inhibition rates of ACE for static desorption and dynamic desorption were 46.09% and 52.1%.Conclusion This process can provide technical references for large-scale industrial production and product development.