不同来源沙门氏菌耐药性及相关性研究

Study on Antimicrobial Resistance with Correlation of Salmonella from Different Sources

中文关键词:  沙门氏菌  血清型  耐药性  耐药基因  Pearson相关性

英文关键词:Salmonella  Serotype  Resistance  Resistance gene  Pearson correlation

中文摘要:

      目的 分析不同来源沙门氏菌的药物敏感性以及耐药基因的携带情况,为沙门氏菌的临床用药以及疾病的防治提供科学依据。方法 对261株来源于人和动物的沙门氏菌进行血清分型,对15种抗生素：氨苄西林、阿莫西林、头孢噻肟、头孢西丁、头孢吡肟、头孢哌酮、头孢曲松、庆大霉素、卡那霉素、阿米卡星、萘啶酸、环丙沙星、磺胺甲恶唑、氯霉素和四环素进行药敏试验,同时测定blaTEM、blaOXA、blaCTX-M、sul1、aacC4、aac(6’)-Ib、floR、tet(A)和tet(G)9种耐药基因的携带情况,并采用卡方及相关性统计学方法分析数据。结果 261株沙门氏菌分为21种血清型,其中肠炎沙门氏菌、印第安纳沙门氏菌、鼠伤寒沙门氏菌和德尔卑沙门氏菌为主要血清型。沙门氏菌对萘啶酸、氨苄西林、四环素耐药率较高,分别为70.9%、55.9%和53.3%,而耐3种及以上抗生素的菌株占63.2%。人源与动物源沙门氏菌耐药情况的Pearson相关性系数为0.973。9种耐药基因中,blaTEM检出率最高为51.3%,其次为tet(A)、blaCTX-M和sul1,分别为33.3%、29.1%与27.2%。结论 沙门氏菌耐药情况严重,耐药谱复杂以及耐药基因携带率较高。多重耐药性菌株较多,且人源与动物源菌株间耐药特征存在明显的相关性。控制沙门氏菌耐药性趋势的发展,应从临床和畜牧业两方面引导抗生素的合理使用。

英文摘要:

      ABSTRACT: Objective To analyzed antimicrobial resistance and the status of resistance genes and provide a scientific basis for the clinical and the prevention of salmonellosis. Methods 261salmonella strains from human were tested for serological types, resistance to 15 kinds of antibiotics (AMP, AMC, CTX, FOX, FEP, CFP, CRO, GN, K, AK, NA, CIP, SXT, C, TE) with the Kirby-Bauer diffusion and 9 resistance genes(blaTEM, blaOXA, blaCTX-M, sul1, aacC4, aac(6’)-Ib, floR, tet(A) and tet(G)),results were analyzed with chi-square and correlation statistics. Results There were 21 serotypes among 261 strains, of which S.enteritidis, S.indiana, S.typhimurium and S.derby were the predominant serotypes. The strains showed highly resistance to nalidixic acid, ampicillin and tetracycline with 70.9%, 55.9% and 53.3%, respectively. The resistance 3 or more antibiotics got to 63.2%. The Pearson correlation coefficient with the resistance from human and animal-derived strains was 0.973. Four resistant genes blaTEM, tet (A), blaCTX-M and sul1 were high carried with 51.3%, 33.3%, 29.1% and 27.2% respectively. Conclusion The status of Salmonella resistance, especially multi-drug resistance, was more serious with highly carried resistant genes. There was a clear correlation between resistance characteristics of human and animal-derived strains. It was necessary for controlling the resistance trends, giving guide to the use of antibiotics in clinical treatment and husbandry.