超高效液相色谱-串联质谱法同时检测功能 食品中6 种玛咖酰胺

Simultaneous determination of 6 kinds of Maca amides in functional foods by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  玛咖  玛咖酰胺  功能性食品  超高效液相色谱-串联质谱法

英文关键词:Maca  Maca amides  functional foods  ultra performance liquid chromatography-tandem  mass spectrometry

中文摘要:

      目的 建立功能性食品中6 种玛咖酰胺类物质的超高效液相色谱-串联质谱同时分析方法。方法 样品经乙醚提取, 氮气吹干, 乙腈定容, Acquity UPLC BEH C18(2.1 mm×100 mm, 1.7 μm)色谱柱分离, 在正离子模式下以电喷雾电离多反应监测模式进行定性和定量分析。结果 本方法在15 min 内完成6 种目标化合物的分离分析。6 种玛咖酰胺在0.1~2 μg/L 范围内线性相关系数r>0.999, 6 种玛咖酰胺检出限均为0.2 μg/kg; 各玛咖酰胺在3 个浓度水平的加标回收率在74.5%~87.2%之间, 相对标准偏差(relative standard deviation, RSD)在2.6%~6.3%之间(n=6)。结论 该方法准确、可靠, 适用于功能性食品中玛咖酰胺的检测。

英文摘要:

      Objective To establish a method for simultaneous determination of 6 kinds of Maca amides in functional foods by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Methods The samples were extracted with ether, dried by nitrogen and made up to the desired volume with acetonitrile. The analytes were separated using Acquity UPLC BEH C18(2.1 mm×100 mm, 1.7 μm) chromatographic column with isocratic elution of acetonitrile/water. Finally, the eluents were qualitatively and quantitatively determined by mass spectrometer with electrospray ionization in positive multiple reaction monitoring mode. Results Six kinds of target compounds were separated and analyzed in 15 min by this method. The 6 kinds of Maca amide had good linear relationships in the range of 0.1~2 μg/L with correlation coefficients more than 0.999. The limits of detection of six kinds of Maca amides were 0.2 μg/kg. The recoveries of Maca amides at 3 spiked levels were 74.5%~87.2%, and the relative standard deviations (RSDs) were 2.6%~6.3% (n=6). Conclusion The method is accurate and reliable, which is suitable for the determination of Maca amides in functional foods.