基于茶汤中应用的单宁酶固定化工艺研究

Tannase immobilization technology based on the application in tea infusion

中文关键词:  壳聚糖  固定化  单宁酶

英文关键词:chitosan  immobilization  tannase

中文摘要:

      目的 优化单宁酶固定方法, 改善茶汤质量。方法 采用壳聚糖、离子交换树脂和活性炭等易得原料作为载体对单宁酶进行固定化研究, 最终确定用壳聚糖作为载体, 采用吸附法制备固定化单宁酶。通过单因素和正交实验对壳聚糖作为载体制备单宁酶的条件进行优化。结果 壳聚糖作为载体固定单宁酶的最佳条件为酶总活力80 U, 吸附时间4 h, 酶与载体吸附温度35 ℃, 制得的固定化单宁酶酶活力回收最高。采用固定化单宁酶对茶汤进行处理并进行感官审评, 经固定化单宁酶处理的茶汤和未经固定化单宁酶处理的茶汤在感官质量上有很大的差异。结论 经固定化单宁酶处理的茶汤质量有很大改善, 澄清效果显著。

英文摘要:

      Objective To optimize the method of tannase immobilization and improve the quality of tea soup. Methods Chitosan, ion exchange resins and activated carbon accessible raw materials were used as carriers for immobilized tannin enzyme research, finally immobilized tannins were prepared by adsorption method with chitosan as the carrier. The conditions of chitosan were optimized by single factor and orthogonal experiment. Results Optimized conditions for the immobilization of tannase were as follows: total enzyme activity was 80 U, adsorption time was 4 h, and adsorption temperature was 35 ℃. The immobilized tannin enzyme activity was the highest under these conditions. The tea soup was processed by immobilized tannase and evaluated with sensory evaluation. The tea soup treated with immobilized tannase and without immobilized tannase had a great difference in sensory quality. Conclusion The quality of tea soup treated by immobilized tannase is improved greatly, and the clarification effect is significant.