高效液相色谱-蒸发光散射检测法同时测定食品中9种甜味剂

Simultaneous determination of 9 kinds of sweeteners in food by high performance liquid chromatography-evaporative light scattering detection

中文关键词:  食品  甜味剂  高效液相色谱法  蒸发光散射检测

英文关键词:food  sweeteners  high performance liquid chromatography  evaporative light scattering detection

中文摘要:

      目的 建立高效液相色谱-蒸发光散射检测器(high performance liquid chromatography-evaporative light scattering detection, HPLC-ELSD)同时检测食品中安赛蜜、糖精钠、甜蜜素、三氯蔗糖、阿斯巴甜、阿力甜、索马甜、纽甜、甜菊糖苷9种甜味剂的方法。方法 样品中的甜味剂经水提取后利用固相萃取柱净化浓缩, 以C18(250 mm×4.6 mm, 5 μm)柱分离, 经流动相梯度洗脱后, 采用HPLC-ELSD法进行测定。结果 9种甜味剂在20~500 mg/L的质量浓度范围内, 具有良好的线性关系(相关系数大于0.99), 在3个添加水平下样品的平均回收率为87.2%~107.1%, 相对标准偏差小于4.0%, 该方法中三氯蔗糖、阿斯巴甜、阿力甜、索马甜、纽甜和甜菊糖苷的检出限为5 mg/kg, 安赛蜜、糖精钠和甜蜜素的检出限为10 mg/kg。结论 本方法简单、准确、灵敏, 是同时检测食品中9种甜味剂的有效方法。

英文摘要:

      Objective To establish a method for simultaneous determination of 9 kinds of sweeteners (acesulfame-K, saccharin sodium, sodium cyclamate, sucralose, aspartame, alitame, thaumatin, neotame and stevioside) in food by high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD). Methods The sweeteners were extracted by water. The extract of sample was cleaned up and concentrated with solid phase extraction cartridge. The sweeteners were separated on a C18 column (250 mm×4.6 mm, 5 μm) and determined by HPLC-ELSD after gradient elution with mobile phase. Results The linear range of the method was 20~500 mg/L with the linear correlation coefficients above 0.99. The average recoveries were from 87.2% to 107.1% at 3 spiked levels and the relative standard deviations were lower than 4.0%. The limits of detection were 5 mg/kg for sucralose, aspartame, alitame, thaumatin, neotame and stevioside, and 10 mg/kg for acesulfame-K, saccharin sodium and sodium cyclamate, respectively. Conclusion This method is simple, accurate and sensitive, which is an effective method for simultaneous determination of 9 kinds of sweeteners in food.