气液微萃取仪联用气相色谱-质谱仪检测果蔬中农药残留量

Detection of pesticide residues in fruits and vegetables by gas-liquid microextraction combined with gas chromatography-mass spectrography

中文关键词:  农药残留  水果  蔬菜  气液微萃取仪  气相色谱质谱仪

英文关键词:pesticide residues  fruits  vegetables  gas-liquid microextraction  gas chromatography-mass spectrometry

中文摘要:

      目的 建立气液微萃取仪(gas-liquid microextraction, GLME)与气相色谱质谱联用仪(gas chromatography- mass spectrography, GC-MS)测定食品中农药残留。方法 称取0.1 g匀浆样品直接加入GLME中进行萃取净化, 定容至100 μL, 结合内标法通过GC-MS检测分析。结果 30种农药在0.001~1 mg/kg浓度范围内线性良好, 相关系数在0.9951~0.9999, 检出限为0.001~0.021 mg/kg, 定量限为0.003~0.070 mg/kg, 平均回收率为67%~119%(n=3), 相对标准偏差在1%~26%之间。 结论 该方法快速、准确, 能满足果蔬类中多种农药残留的定性定量分析。

英文摘要:

      Objective To establish a method for the determination of pesticide residues in fruits and vegetables by gas-liquid microextraction (GLME) and gas chromatography-mass spectrometry (GC-MS). Method The 0.1 g of homogeneous sample was directly added into GLME for extraction and purification. The volume was adjusted to 100 μL and the samples were analyzed by GC-MS with internal standard method. Result The 30 kinds of pesticide had good linear relationships in the range of 0.001~1 mg/kg, and the correlation coefficients were 0.9951~0.9999. The limits of detection were 0.001~0.021 mg/kg and the limits of quantitation were 0.003~0.070 mg/kg. The average recoveries were 67%~119% (n=3) with relative standard deviations of 1%~26%. Conclusion The method is fast and accurate, which can satisfy the quantitative analysis of various pesticide residues in fruits and vegetables.