进境多肉植物细菌性褐腐病的病原分离与鉴定

Isolation and identification of brown rot bacteria on imported succulent plants

中文关键词:  多肉植物  细菌性褐腐病  病原鉴定  菠萝泛菌

英文关键词:succulent plants  brown rot bacteria  pathogen identification  Pantoea ananatis

中文摘要:

      目的 通过对病变叶片进行细菌的分离鉴定, 明确韩国进境多肉植物上叶片褐色腐烂病斑的病原。 方法 采用组织分离法, 从韩国进境多肉植物叶片中分离纯化到1株细菌菌株, 对该菌株进行了鉴定和生物学特性研究。结果 形态及培养特征、生理生化特性的研究结果表明该菌株在营养琼脂(nutrient agar, NA)培养基上单菌落圆形淡黄色, 边缘整齐。菌体直杆状, 周生鞭毛运动, 无芽孢, 为革兰氏阴性菌。经形态鉴定、培养特征、生理生化、致病性测定及16S rDNA序列分析, 确认引起该多肉植物发生褐腐病的病原是菠萝泛菌(Pantoea ananatis)。结论 经过对发生细菌性褐腐病的叶片进行病原分离与鉴定, 有效阻止了菠萝泛菌的远距离传播。

英文摘要:

      Objective To make clear pathogens of brown rot bacteria in succulent plants imported from Korea by isolation and identification of bacteria. Methods One strain of pathogenic bacteria was isolated from imported Korea succulent plants leaves by tissue isolation method. The strain was identified and biological characteristics were studied. Results Morphological characteristics, culture patterns and physiological and biochemical reactions results indicated that all strains formed pale yellow, glossy, and smooth, round single colony on nutrient agar (NA). The bacterial cells were gram-negative rods with peritrichous flagellum under the microscope. According analysis results of the pathogenicity, staining reactions, morphological characteristics, culture patterns, physiological and biochemical reactions, and 16S rDNA gene sequences, the isolated strain was identified as Pantoea ananatis. Conclusion Through isolation and identification of pathogen of bacterial brown rot, it effectively prevents the long distance spread of Pantoea ananatis.