超高效液相色谱法同时测定饮料中14种 食品添加剂

Simultaneous determination of 14 kinds of food additives in beverage by ultra performance liquid chromatography

中文关键词:  超高效液相色谱法  饮料  着色剂  甜味剂  防腐剂

英文关键词:ultra performance liquid chromatography  beverage  coloring agent  sweetener  antiseptic

中文摘要:

      目的 建立一种应用超高效液相色谱法同时测定饮料中14种着色剂、甜味剂和防腐剂的分析方法。方法 样品经ZOXBAX SB-C18色谱柱分离, 以甲醇和100 mmol/L磷酸二氢铵溶液为流动相进行梯度洗脱, 流速0.2 mL/min, 柱温35 ℃。采用二极管阵列检测器, 在190~600 nm波长下进行扫描, 230 nm和200 nm波长下进行检测, 外标法定量。结果 在最佳色谱条件下, 这14种食品添加剂可在20 min内有效分离, 标准曲线线性关系良好(r＞0.996)。平均回收率为92.7%~97.1%, 相对标准偏差为0.61%~2.54%。当取样量为5 g, 稀释体积为25 mL时, 检出限(limits of detection, LODs)为0.3~0.6 mg/kg。结论 建立的方法可实现对饮料中多种着色剂、甜味剂和防腐剂的同时检测, 具有快速、准确、灵敏的特点。

英文摘要:

      Objective To establish a method for simultaneous determination of 14 kinds of coloring agents, sweeteners and antiseptics in beverage by ultra performance liquid chromatography (UPLC). Methods The samples were separated by Agilent ZORBAX SB-C18 column with mobile phase consisting of methanol and ammonium dihydrogen phosphate solution (100 mmol/L). The gradient elution was at a flow rate of 0.2 mL/min and column temperature was 35 ℃. These food additives were detected by diode array detector in the wavelength range from 190 to 600 nm with detection wave length at 230 nm and 200 nm. The detection results were quantitated by an external standard method. Results Under the optimal conditions, 14 kinds of food additives could be effectively separated within 20 min. The standard curves had good linearities (r＞0.996). The average recoveries were in the range of 92.7% to 97.1% and the relative standard deviations (RSDs) were 0.61%~2.54%. The limits of detection (LODs) of 14 kinds of food additives were 0.3~0.6 ng. Conclusion The established method is rapid, accurate and sensitive, which is suitable for simultaneously detection of various coloring agents, sweeteners and antiseptics in beverage.