海南产苦丁茶主要营养品质评价与酚类物质组成分析

Evaluation of primary nutritional quality and analysis of polyphenols compositions of Hainan Ilex latifolia Thunb

中文关键词:  海南苦丁茶  营养评价  抗氧化  高效液相色谱-质谱法  酚类

英文关键词:Hainan Ilex latifolia Thunb  nutritional evaluation  antioxidant activity  high performance liquid chromatography-mass spectrometry  phenols

中文摘要:

      目的 对海南苦丁茶主要营养成分和抗氧化活性成分进行分析, 为其品质评价和综合加工利用提供基础数据。方法 对海南产苦丁茶中主要营养成分(氨基酸、脂肪酸及矿物质元素)的组成和含量进行测定, 并利用高效液相色谱-质谱联用技术(high performance liquid chromatography-mass spectrometry, HPLC-MS)对抗氧化活性较高的70%乙醇提取物中的特征性成分进行分析。结果 海南产苦丁茶中的主要营养成分为碳水化合物和粗脂肪; 其常量与微量元素含量较为丰富, 以Mg、Ca和Mn含量相对较高。富含人体必需氨基酸和不饱和脂肪酸, 氨基酸组成合理均衡。通过 HPLC-MS从70％乙醇提取物中鉴定出新绿原酸(5-咖啡酰奎宁酸)、绿原酸(3-咖啡酰奎宁酸)、隐绿原酸(4-咖啡酰奎宁酸)、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸等6种酚类物质。结论 海南产苦丁茶具有较好的食用价值和保健作用。

英文摘要:

      Objective To analyze the primary nutritional compositions and antioxidant constituents of Hainan Ilex latifolia Thunb and provide basic data for its quality evaluation and comprehensive processing and utilization. Methods Primary nutritional compositions (amino acids, fatty acids and mineral elements) of Ilex latifolia Thunb were measured and analyzed. In addition, the characteristic constituents of 70% ethanol extract with higher antioxidant activity were analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS). Results The main nutrients in Hainan Ilex latifolia Thunb were carbohydrate and crude fat. The content of macroelements and trace elements were relatively abundant with higher content of Mg, Ca and Mn. It had a well-balanced amino acid composition with rich in essential amino acids and unsaturated fatty acids. Totally 6 kinds of major phenols were identified by HPLC-MS, including neochlorogenic acid (5-caffeoylquinic acid), chlorogenic acid (3-caffeoylquinic acid), cryptochlorogenic acid (4-caffeoylquinic acid), 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid. Conclusion Ilex latifolia Thunb has good edible value and health care function.