| 李化强,吴菲菲,龙艳珍,赵良忠,徐永平,李淑英.微切助互作技术提取龙牙百合鳞茎总黄酮工艺研究[J].食品安全质量检测学报,2017,8(8):3074-3079 |
| 微切助互作技术提取龙牙百合鳞茎总黄酮工艺研究 |
| Extraction of total flavonoids by press-shear assisted interaction technology from Longya lilium bulb |
| 投稿时间：2017-06-13 修订日期：2017-08-15 |
| DOI： |
| 中文关键词: 龙牙百合鳞茎 微切助互作技术 总黄酮 微晶纤维素 |
| 英文关键词:Longya lilium bulb press-shear assisted interaction technology total flavonoids microcrystalline cellulose |
| 基金项目:国家科技部863计划项目(2013AA102805-03)、湖南省果蔬清洁加工工程技术研究中心项目(2015TP2022)、湖南省自科基金青年项目(2017JJ3284)、湖南省教育厅优秀青年项目(17B241)、湖南省教育厅一般项目(17C1433) |
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| Author | Institution |
| LI Hua-Qiang | College of Food and Chemistry Engineering, Shaoyang University, Hunan Provincial Engineering and Technology Research Center for Fruit and Vegetable Clean Processing, School of Life Science and Biotechnology, Dalian University of Technology, Postdoctoral Research Stations of Dalian SEM Bio-Engineering Technology Co., Ltd. |
| WU Fei-Fei | College of Food and Chemistry Engineering, Shaoyang University, Hunan Provincial Engineering and Technology Research Center for Fruit and Vegetable Clean Processing, School of Life Science and Biotechnology, Dalian University of Technology, Postdoctoral Research Stations of Dalian SEM Bio-Engineering Technology Co., Ltd. |
| LONG Yan-Zhen | College of Food and Chemistry Engineering, Shaoyang University, Hunan Provincial Engineering and Technology Research Center for Fruit and Vegetable Clean Processing |
| ZHAO Liang-Zhong | College of Food and Chemistry Engineering, Shaoyang University, Hunan Provincial Engineering and Technology Research Center for Fruit and Vegetable Clean Processing |
| XU Yong-Ping | School of Life Science and Biotechnology, Dalian University of Technology, Postdoctoral Research Stations of Dalian SEM Bio-Engineering Technology Co., Ltd. |
| LI Shu-ying | Postdoctoral Research Stations of Dalian SEM Bio-Engineering Technology Co., Ltd. |
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| 中文摘要: |
| 目的 利用微切助互作技术优化龙牙百合鳞茎提取总黄酮的工艺方法。方法 采用微晶纤维素为助剂, 龙牙百合鳞茎为主要原料, 总黄酮提取率为指标, 通过单因素和正交试验优化龙牙百合鳞茎总黄酮的最优提取参数。结果 总黄酮提取工艺条件为: 微晶纤维素添加量1.5%(m:m), 提取时间35 min, 提取温度50 ℃, 乙醇体积分数60%(V:V)。该条件下总黄酮提取率为5.98%, 是热回流法的2.26倍, 且提取总时长由122 min缩短到62 min, 以60%乙醇作提取溶剂, 大大减少了有机溶剂使用。结论 微切助互作技术是提取龙牙百合鳞茎总黄酮的一种高效、低成本方法, 具有很好的应用前景。 |
| 英文摘要: |
| Objective To optimize production process of total flavonoids extraction from Longya lilium bulb by press-shear assisted interaction (PAI) technology. Methods The optimum parameters of total flavonoids were investigated using microcrystalline cellulose as additive, Longya lilium bulb as materials, and total flavonoids yield as an index in single factor test and orthogonal test. Results The extraction conditions of total flavonoids were as follows: microcrystalline cellulose dosage was 1.5% (m:m), extraction time was 35 min, extraction temperature was 50 ℃ and ethanol volume fraction was 60% (V:V). Under the optimal conditions, the total flavonoids of Longya lilium bulb was up to 5.98%, which was 2.26 times as that of hot reflux, the total time was shortened from 122 min to 62 min, and the use of organic solvents was greatly reduced. Conclusion PAI technology exerts an efficient and low-cost method for extraction of total flavonoids from Longya lilium bulb, which has a good application prospect. |
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