浊点萃取-纳米金探针联用可视化检测鱼肉中的Hg2+

Visual detection of mercury (Hg2+) in fish by cloud point extraction-gold nanoparticle probe

中文关键词:  浊点萃取  纳米金探针  可视化检测  汞  鱼

英文关键词:cloud point extraction  gold nanoparticle probe  visual detection  mercury  fish

中文摘要:

      目的 建立一种浊点萃取与纳米金探针联用快速可视化检测鱼肉中Hg2+的方法。方法 修饰的纳米金粒子与Hg2+特异性络合, 经浊点萃取进入有机相, 并出现可肉眼观察的红色沉淀。根据有机相中沉淀量的多少或颜色深浅可判定Hg2+的浓度。结果 Hg2+浓度在1~15 μg/L范围内, 沉淀量和沉淀颜色与溶液中的Hg2+浓度呈正相关。本方法肉眼可识别的Hg2+浓度最低为2 μg/L。结论 本方法对Hg2+选择性好、灵敏度较高、检测时间短、操作简便, 可用于鱼肉中Hg2+的快速定性分析。

英文摘要:

      Objective To establish a visual detection method for tract mercury (Hg2+) in real fish samples, based on the cloud point extraction and gold nanoparticle (AuNP) probe techniques. Methods Modified and purified AuNP was specifically complexed with Hg2+ in the basified digestion solution of fish sample. The complex was then extracted by cloud point extraction and the red deposits were appeared in the Triton X-114. According to the amount or color of precipitation in organic phase, Hg2+ concentration could be determined. Results There was a positive correlation of the concentration of Hg2+ and amount of red deposit in the range of 1~15 μg/L Hg2+ in the basified digestion solution of fish samples. The lowest concentration which could be distinguished by naked eye was 2 μg/L. Conclusion This method is rapid, efficient and convenient with good selectivity and high sensitivity, which can be used for rapid qualitative analysis of Hg2+ in fish samples.