温室内土壤-气溶胶中四环素类抗生素抗性基因的分布特征

Distribution of tetracycline antibiotic resistance genes in soil-aerosols in greenhouse

中文关键词:  气溶胶  土壤  四环素类抗生素抗性基因  温室

英文关键词:aerosol  soil  tetracycline antibiotic resistance gene  greenhouse

中文摘要:

      目的 研究温室内四环素类抗生素抗性基因(antibiotic resistance genes, ARGs)在土壤和气溶胶中的分布特征。方法 以日光温室为研究对象, 通过对温室内土壤、气溶胶样品中的17种四环素类ARGs进行实时荧光定量PCR测定, 分析ARGs在土壤样品、不同切割粒径下的气溶胶样品中的分配分布特征。结果 研究显示土壤及气溶胶中Tet(32s)检出率为100%, 其次是Tet(A)、Tet(W)。随着气溶胶采样的切割粒径变小, 检出的四环素类ARGs种类增加, 丰度提高。PM2.5中的Tet(32s)平均丰度分别是土壤、TSP、PM10中的571.8倍、4.66倍和0.80倍。细胞保护是温室内四环素类ARGs的主要抗性机制。结论 气溶胶和土壤均为ARGs的储库, 不同切割粒径的气溶胶样品中ARGs的富集程度差别较大。鉴于气溶胶ARGs流动扩散能力强, 比土壤ARGs的潜在风险更大, 因此气溶胶ARGs亟待深入研究。

英文摘要:

      Objective To investigate and analyze the distribution of antibiotic resistance genes (ARGs) in soil and aerosol in greenhouse. Methods A total of 17 tetracycline ARGs in soil and aerosol samples from greenhouse as the research object were measured by real-time fluorescence quantitative PCR. The distributions of ARGs in the soil and different cutting particle sizes of aerosol were analyzed. Results The detection rates of Tet (32s) in soil and aerosol were 100%, followed by Tet (A) and Tet (W). With the decrease of the size of aerosol samples, the detection and abundance of tetracycline ARGs increased. The average abundance of Tet (32s) in PM2.5 was 571.8-fold, 4.66-fold and 0.80-fold that of soil, TSP and PM10, respectively. Cell protection was the main resistance mechanism of tetracycline ARGs in greenhouse. Conclusion Aerosols and soils are both ARGs reservoirs, and the enrichment of ARGs in aerosol samples with different sizes is quite different. In view of diffusion ability of aerosol ARGs, it is higher potential risk than the soil ones. Aerosol ARGs need to be studied further.