大鲵低聚糖肽的研究进展

Research progress of Andrias davidianus glcopetides

中文关键词:  大鲵低聚糖肽  黏液  鉴定  应用

英文关键词:Andrias davidianus glcopetides  mucus  identification  application

中文摘要:

      大鲵体表黏液是一种有重要应用价值的生物资源。使用海洋微生物酸性蛋白酶(Aspergillus sp. acid protease)以及膜分离、纳滤膜除盐等步骤,从大鲵体表黏液中制备出了分子量小于3500Da的大鲵低聚活性糖肽。β-消旋反应、血凝集抑制试验结果表明大鲵低聚糖肽具有O-连接糖肽键。大鲵低聚糖肽具有促进小鼠免疫功能的作用、体外抗氧化活性、抗疲劳作用、抗紫外线能力以及对CCl4导致小鼠肝损伤具有保护作用。因此,大鲵低聚糖肽在食品、保健食品以及药品领域有着广泛的潜在应用。

英文摘要:

      The mucus of Andrias davidianus is one of important and valuable biological source. By using Aspergillus sp. acid protease, membrane separation and nanofiltration membrane, A. davidianus glcopetides (ADGPs) with 3500 Da from mucus was prepared. According to β-elimination reaction and hemagglutination, the linkage between the polysaccharide moiety and peptide of glycopeptides might belong to O-glucosidic linkage. The immunological function of mice, antioxidant activity in vitro, anti-fatigue, anti-uv-ray and hepatoprotective effects were improved by ADGPs. Therefore, ADGPs have potential application in food, health food and drug field.