超高液相色谱-串联质谱法测定猪肉中8种喹诺酮类兽药残留的不确定度评定

Uncertainty evaluation of the determination of 8 kinds of quinolones residues in pork by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  超高液相色谱-串联质谱法  喹诺酮  不确定度  猪肉

英文关键词:ultra performance liquid chromatography-tandem mass spectrometry  quinolones  uncertainty  pork

中文摘要:

      目的 评定超高液相色谱-串联质谱法测定猪肉中8种喹诺酮类兽药残留量的不确定度。方法 根据JJF 1135-2005《化学分析测量不确定度评定》和JJF 1059.1-2012《测量不确定度评定与表示》中的有关规定, 建立不确定度的数学模型, 逐层对不确定度进行分析。结果 添加量为1.0 μg/kg时, 8种喹诺酮类兽药残留量测定的相对标准不确定度为2.77%~6.65%; 添加量为2.0 μg/kg时, 8种喹诺酮类兽药残留量测定的相对标准不确定度为1.69%~6.67%; 添加量为4.0 μg/kg时, 8种喹诺酮类兽药残留量测定的相对标准不确定度为1.31%~5.43%。结论 在实际检测中, 提高天平精度及定容精度, 控制标准溶液配制过程, 提高检验员工作质量和效率均可降低引入的不确定度, 使检测结果更可靠。

英文摘要:

      Objective To evaluate the uncertainty of measurement in the determination of 8 kinds of quinolones residues in pork by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods According to JJF 1135-2005 Evaluation of Uncertainty in Chemical Analysis Measurement and JJF 1059.1-2012 Evaluation and Expression of Uncertainty in Measurement, a mathematical model was established for uncertainty evaluation. The sources of uncertainty were analyzed. Results When the addition was 1.0 μg/kg, the relative standard uncertainties of the 8 kinds of quinolones residues were 2.77%~6.65%; When the addition was 2.0 μg/kg, the relative standard uncertainties of the 8 kinds of quinolones residues were 1.69%~6.67%; When the addition was 4.0 μg/kg, the relative standard uncertainties of the 8 kinds of quinolones residues were 1.31%~5.43%. Conclusion In actual detection, the increase of balance accuracy and constant volume accuracy, control of the standard solution calibration process and improving the quality and efficiency of testers can reduce the uncertainty of measurement results.