超高效液相色谱法测定化妆品中对羟基苯乙酮含量

Determination of 4-hydroxyacetophenone in cosmetics by ultra performance liquid chromatography

中文关键词:  对羟基苯乙酮  超高效液相色谱法  化妆品

英文关键词:4-hydroxyacetophenone  ultra performance liquid chromatography  cosmetic

中文摘要:

      目的 建立超高效液相色谱法(ultra performance liquid chromatography, UPLC)测定化妆品中对羟基苯乙酮含量的分析方法。方法 样品前处理以甲醇为溶剂, 超声30 min, 采用色谱柱Atlantis? d C18分离, 以甲醇-水(25:75, V:V)为流动相, 流速为0.2 mL/min, 检测波长为275 nm, 经UPLC检测; 同时对提取剂和提取时间、流动相比例、检测波长等条件进行优化。结果 对羟基苯乙酮浓度在0.4376~21.83 μg/mL范围内呈良好的线性关系, 相关系数为0.9996, 膏霜、乳、液等不同剂型样品回收率高, 平均回收率分别为99.89%、99.83%和100.24%, RSD分别为0.75%、0.65%和0.46%。方法检出限为1.38 μg/g。结论 该方法简单、快速、准确, 可适用于化妆品中对羟基苯乙酮含量的测定。

英文摘要:

      Objective To establish a method for the determination of 4-hydroxyacetophenone in cosmetics by ultra performance liquid chromatography (UPLC). Methods Samples were extracted with methanol by ultrasonic extraction for 30 min, and separated by an Atlantis? d C18 column with methanol-water (25:75, V:V) as mobile phase at the flow rate of 0.2 mL/min, and then detected by UPLC under the detection wavelength of 275 nm. Meanwhile, extraction solvent and extraction time, mobile phase ratio and detection wavelength were optimized. Results 4-Hydroxyacetophenone had a good linear relationship in the range of 0.4376~21.83 μg/mL with the correlation coefficient of 0.9996. The recovery rates of different dosage forms such as paste, emulsion and liquid preparation were high. The average recoveries of paste, emulsion and liquid preparation were 99.89%, 99.83% and 100.24%, and relative standard deviations (RSDs) were 0.75%, 0.65% and 0.46%, respectively. The limit of detection was 1.38 μg/g. Conclusion The method is simple, rapid and accurate, which can be used for the determination of 4-hydroxyacetophenone in cosmetics.