高效液相色谱法检测保健食品中的番茄红素

Determination of lycopene in health food by high performance liquid chromatography

中文关键词:  保健食品  番茄红素  高效液相色谱法

英文关键词:healthy food  lycopene  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定保健食品中番茄红素的分析方法。方法 用含有0.1% 2,6-二叔丁基-4-甲基苯酚抗氧化剂的甲醇:二氯甲烷(10:90, V:V)作为提取溶液。通过Agilent Eclipse XDB-C18(4.6 mm×250 mm, 5 μm)色谱柱; 甲醇:乙酸乙酯(85:15, V:V)作为流动相, 二极管阵列检测器, 检出波长475 nm, 色谱柱温25 ℃, 流速1.0 mL/min, 进样体积10 μL, 作为检测条件。结果 番茄红素在0~200 μg/mL范围内线性良好, 相关系数r=0.9995, 最低检出限为0.0815 μg/mL, 平均回收率在89.7%~109.8%之间, RSD为8.4%。结论 本方法可以准确、快速地检测保健食品中的番茄红素。

英文摘要:

      Objective To establish a method for determination of lycopene in health food by high performance liquid chromatography (HPLC). Methods Mixed solution of methyl alcohol: dichloromethane (10:90, V:V) with 0.1% 2,6-di-tert-butyl-4-methylphenol (BHT) antioxidants were used as extraction solutions. The extracting solutions were injected into HPLC system equipped with a diode array detector (DAD) at the absorption wavelength of 475 nm, with an Agilent Eclipse XDB-C18 reverse phase column (4.6 mm×250 mm, 5 μm), which was kept at 25 ℃. The mobile phase was composed of 85% methanol and 15% ethyl acetate at a flow rate of 1.0 mL/min. The injection volume was 10 μL. Results The linearity range was 0~200 μg/mL (r=0.9995), the limit of detection (LOD) was 0.0815 μg/mL, and the average recoveries were between 89.7% and 109.8% with RSD of 8.4%. Conclusion This method can accurately and quickly determine the lycopene in healthy food.