微波消解氢化物原子荧光光谱法测定食品中的硒

Microwave digestion hydride atomic fluorescence spectrometry for determination of selenium in food

中文关键词:  微波消解  氢化物原子荧光光谱法  硒

英文关键词:microwave digestion  hydride atomic fluorescence spectrometry  selenium

中文摘要:

      目的 优化微波消解-氢化物原子荧光光谱法检测食品中的微量元素硒。方法 采用不同前处理方法对检测结果影响进行研究, 分别对质控样经微波消解后是否赶酸作对比, 赶酸时是否加盐酸作对比, 微波消解与电热板湿法消解作对比, 用氢化物原子荧光光度仪测定其含量。结果 用微波消解, 赶酸后加盐酸继续赶酸, 用原子荧光光谱仪进行检测, 检出限为0.425 μg/kg, 定量限为1.42 μg/kg, 精密度为2.67%, 回收率为93%~109%。结论 该方法简便、快速、准确度高, 可用于食品中硒的检测。

英文摘要:

      Objective To optimize the method for determination of microelement selenium in food by microwave digestion hydride generation atomic fluorescence spectrometry. Methods The effects of different pretreatment methods on detection results were studied. Through quality control samples after microwave digestion and acid-driving, adding hydrochloric acid and acid-driving without HCl, microwave digestion and electric heating plate digestion were compared, respectively, and its content was determined by atomic fluorescence spectrometry. Results With microwave digestion, after acid-driving, hydrochloric acid was added to drive the acid, and the atomic fluorescence spectrometer was used to detect samples. The limit of detection was 0.425 μg/kg, and limit of quantitation was 1.42 μg/kg. The precision of the method was 2.67%, and the recovery rates were 93%~109%. Conclusion The method is simple, rapid and accurate, which can be used for the detection of selenium in food.