高效液相色谱法测定槟榔中阿斯巴甜的含量

Determination of aspartame in areca by high performance liquid chromatography

中文关键词:  槟榔  阿斯巴甜  高效液相色谱法

英文关键词:areca  aspartame  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法检测槟榔中阿斯巴甜的含量的分析方法。方法 槟榔去除卤水及内层纤维后, 以磷酸-水溶液(pH=2)为提取液、采用超声法提取槟榔中阿斯巴甜。提取液经Agilent ZORBAX SB-C18(4.6 mm×250 mm, 5 μm)色谱柱分离, 以乙腈-10 mmol/L KH2PO4水溶液(pH=3.5)(14:86, V:V)为流动相, 流速为1 mL/min, 采用紫外检测器在210 nm检测波长下对阿斯巴甜含量进行测定。结果 阿斯巴甜在4.444~222.2 μg/mL范围内呈现良好的线性关系, 检出限为0.22 μg/mL, 精密度为0.17%, 在3个加标水平下的平均回收率为99.80%。结论 本提取方法易于操作、提取率高且稳定性良好, 可用于槟榔中阿斯巴甜的提取; 本检测方法具有线性范围广、灵敏度高、快速、准确等优点, 适用于槟榔中阿斯巴甜的含量测定。

英文摘要:

      Objective To establish a method for determination of aspartame in areca by high performance liquid chromatography (HPLC). Methods Aspartame was extracted from areca by ultrasound extraction method with phosphoric acid aqueous solutions (pH=2) after removed of brine and inner fiber. The extraction solution was separated by Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5 μm), using acetonitrile and 10 mmol/L KH2PO4 solution (pH=3.5)(14:86, V:V)as mobile phase with a flow rate of 1 mL/min, and detected by ultraviolet detector at the wavelength of 210 nm. Results Aspartame had a good liner relationship in the range of 4.444~222.2 μg/mL, and the limit of detection of aspartame was 0.22 μg/mL. The precision of this method was 0.17% and the average recoveries spiked at 3 levels was 99.80%. Conclusion The extraction method is easy to operate with good extraction yield and good stability, which can be used for extracting aspartame from areca. Besides, the detection method has the advantages of wide linear range, high sensitivity, rapidness and accuracy, which can be used for determination of aspartame in areca.