超高液相色谱串联质谱法检测土豆及土豆制品中α-茄碱

Determination of α-solanine in potato and its products by ultra-high performance liquid chromatography tandem mass spectrometry

中文关键词:  马铃薯  超高效液相色谱串联质谱法  α-茄碱

英文关键词:potato  ultra-high performance liquid chromatography-tandem mass spectrometry  α-solanine

中文摘要:

      目的 建立超高效液相色谱串联质谱法检测土豆及其制品中α-茄碱的分析方法。方法 通过优化提取液, 土豆样品经1%甲酸-甲醇(1:1, V:V)超声提取, 采用BEH C18色谱柱(2.1 mm ×50 mm, 1.7 μm)分离, 梯度洗脱, 采用超高效液相色谱串联质谱法, 在多反应监测模式进行定性和定量分析, 而土豆淀粉用MCX固相萃取柱进行净化, 液相色谱串联质谱法测定。结果 土豆样品中回收率为75%~115%, 相对标准偏差为5.0%~8.6%, 土豆淀粉的回收率范围为67%~100%, 相对标准偏差为3.4%~11.3%, 方法的检出限和定量限分别为0.5 mg/kg和1.0 mg/kg。结论 该方法适合于土豆及其制品中α-茄碱的分析。

英文摘要:

      Objective To establish a method for the determination of α-solanine in potato and its products by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Potato samples were extracted by the mixture of 1% formic acid and methanol (1:1, V:V), and separated by BEH C18 column (2.1 mm×50 mm, 1.7 μm) with gradient elution. Multiple reaction monitoring model was used for qualitative and quantitative analysis. The potato starch was extracted and purified by MCX solid phase extraction and determined by UPLC-MS/MS. Results The recoveries of potato samples were 75%~115% with the relative standard deviation in the range of 5.0%~8.6%. The recoveries of potato starch were 67%~100% with the relative standard deviation in the range of 3.4%~11.3%. The limit of detection and the limit of quantification were 0.5 mg/kg and 1.0 mg/kg, respectively. Conclusion The method is suitable for determination of α-solanine in potato and its potato products.