超高效液相色谱-串联质谱法同时测定食品中麦芽酚、乙基麦芽酚、香兰素、甲基香兰素和乙基香兰素

Simultaneous determination of vanillin, methyl vanillin, ethyl vanillin, maltol and ethyl maltol in foods by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  超高效液相色谱-串联质谱法  麦芽酚  乙基麦芽酚  香兰素  甲基香兰素  乙基香兰素

英文关键词:ultra performance liquid chromatography-tandem mass spectrometry  vanillin  methyl vanillin  ethyl vanillin  maltol  ethyl maltol

中文摘要:

      目的 采用超高效液相色谱-串联质谱法建立同时测定食品中麦芽酚、乙基麦芽酚、香兰素、甲基香兰素和乙基香兰素5种香精的分析方法。方法 样品经甲醇提取, WondaSep MAX固相萃取柱净化。目标化合物以0.1%甲酸-甲醇溶液为流动相, 在梯度洗脱条件下经InertSustain Bio C18(2.1 mm×150 mm, 3 μm)色谱柱分离后采用电喷雾离子源(electrospray ionization, ESI)正离子多反应监测模式(multiple reaction monitoring, MRM)进行检测。结果 5种香精线性相关系数均大于0.99, 方法定量限在0.9~16.2 ng/g之间, 在3个加标水平下的平均回收率为80.1%~106.0%, 相对标准偏差不大于8.9%。结论 该方法准确、灵敏, 可用于液体乳、婴幼儿配方食品、食用油、乳制品、饮料、烘焙食品等实际样品中香兰素、甲基香兰素、乙基香兰素、麦芽酚和乙基麦芽酚的同时测定。

英文摘要:

      Objective To establish a method for simultaneous determination of vanillin, methyl vanillin, ethyl vanillin, maltol and ethyl maltol in foods by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The samples were extracted with methanol, and purified with WondaSep MAX solid phase extraction column. The separation was performed on an InertSustain Bio C18 (2.1 mm×150 mm, 3 μm) column using a gradient elution program with mobile phase of 0.1% formic acid and methanol solution. The 5 kinds of components were determined in the modes of electrospray positive ionization (ESI+) and multiple reaction monitoring (MRM). Results The correlation coefficients of 5 kinds of components were more than 0.99. The limits of quantitation (LOQs) were 0.9~16.2 ng/g. The average recoveries at 3 spiked levels ranged from 80.1%~106.0% with the relative standard deviations (RSDs) less than 8.9%. Conclusion The established method is accurate and sensitive, and suitable for the simultaneous determination of vanillin, methyl vanillin, ethyl vanillin, maltol and ethyl maltol in milk, infant formula food, edible oil, dairy products, beverages and baked goods.