DNA条形码在肉制品掺伪中非定向筛查技术的研究

Research on the non-directional test in meat adulteration based on DNA barcode

中文关键词:  DNA条形码  COI基因  肉制品掺伪  非定向筛查

英文关键词:DNA barcode  COI  meat adulteration  non-directional detection

中文摘要:

      目的 对基于DNA条形码的肉制品掺伪中非定向筛选技术进行探索。方法 以线粒体基因组中COI基因为靶标, 设计猪、牛、羊、马、鸡、鸭、鹅、鼠8种动物源物种间的通用引物和特异性引物, 建立禽畜肉的DNA条形码检测方法。同时应用该技术对50份市售的肉制品进行检测。结果 本研究建立了DNA条形码筛选技术, 电泳条带通过基因测序能正确识别猪、牛、羊、马、鸡、鸭、鹅、鼠8种动物源成分, 结合分子克隆技术能实现对混合肉的检测。50份市售肉制品的DNA条形码结果显示, 16份掺杂了除牛羊肉以外的其他禽畜肉。结论 DNA条形码技术能打破传统标准中PCR法检测目标唯一性的局限, 具有良好的应用前景。

英文摘要:

      Objective To research non-directional test method of meat adulteration based on DNA barcode. Methods Based on COI gene in mitochondria, universal primers and specific primers of pork, beef, mutton, horsemeat, chicken, duck, goose and rat were designed and DNA barcode method was established. And then 50 meat products bought from markets were detected by this method. Results Pork, beef, mutton, horsemeat, chicken, duck, goose and rat meat could be distinguished with each other after sequencing gene of electrophoresis banding in the established DNA barcode method. Combined with molecular cloning technique, mixed meat could be tested by this method. The DNA barcode test results of 50 meat products showed that 16 of them were adulterated with other livestock meat except for beef and mutton. Conclusion DNA barcode can break the limitation of which the target of PCR method is unique in traditional standard, and this technology has the extremely broad application prospect.