高温高压加热受试物对细菌回复突变试验结果的影响

Effect of high temperature and high pressure heating on the tested materials for the bacterial reverse mutation (Ames) test

中文关键词:  细菌回复突变试验  高温高压  致突变作用  影响

英文关键词:bacterial reverse mutation test  high temperature and high pressure  mutagenicity  effect

中文摘要:

      目的 研究高温高压加热灭菌对受试物进行细菌回复突变(Ames)试验, 并评价其致突变作用结果稳定性的影响。方法 以细菌回复突变试验推荐阳性物为研究对象, 使用高压灭菌锅对阳性物进行加热灭菌处理。采用平板掺入法验证加热和未加热阳性物对菌株的诱导回变效率。进一步将推荐阳性物等体积配制为混合阳性物后, 将其掺入到一种以中药颗粒为主的保健食品中配制为模拟阳性受试物, 考察浓度和加热2个条件下混合阳性物的诱导回变效率。结果 加热会明显降低推荐的单一阳性物诱变活力, 混合阳性物在以保健食品为基质中的结果也基本一致, 但受到阳性物浓度和基质浓度的影响。结论 高压灭菌易导致阳性物的诱导效率下降, 对Ames试验有一定程度的影响, 特别是对保健食品的测试准确性具有影响; 而开发新的前处理方法是避免上述问题的关键。

英文摘要:

      Objective To investigate and evaluate the effect of high temperature and high pressure heating and sterilization on the tested materials for the bacterial reverse mutation (Ames) test. Methods The positive compounds recommended by Ames test as research object, were sterilized by high pressure sterilization pot. The efficiency of the induction of heating and non heating positive compounds on bacterial strain was verified by the plate incorporation method. The recommended positive substances were mixed into a mixed positive substance, the mixture were mixed into a kind of health food with Chinese medicine mainly for simulation of particle positive compounds, and the efficiency of the induction of mixed positive compounds under concentration and heating conditions was verified. Results Mutagenic activity of single positive compound recommended was reduced significantly by heating, the results of mixed positive in the health food as the matrix was also basically consistent, but it was affected by the concentration of the positive compounds and concentration of the matrix. Conclusion High pressure sterilization can easily lead to the decrease of the induction efficiency of the positive compounds, and there is a degree of influence on the Ames test, especially the accuracy of health food testing. The key to avoiding these problems is to develop a new pretreatment approach.