小米中姜黄染色的快速检测

Rapid detection of turmeric coloration in millet

中文关键词:  小米  姜黄染色  超高效液相色谱法-二极管阵列检测器

英文关键词:millet  turmeric coloration  ultra performance liquid chromatography-diode array detector

中文摘要:

      目的 建立一种快速检查小米中姜黄染色的检测方法。方法 样品经乙酸-甲醇溶液超声提取后过滤, 滤液蒸干, 经硼酸-草酸溶液显色后检查姜黄染色的存在, 通过超高效液相色谱法-二极管阵列检测器(ultra performance liquid chromatography-diode array detector, UPLC-DAD)对样品的姜黄染色进行定性、定量分析。结果 显色反应具有较高的灵敏性。姜黄素在1.0~20 μg/mL浓度范围(样品含量4~80 mg/kg)时线性关系良好, 方法精密度RSD值小于2%, 方法平均回收率为99.8%。结论 该方法快速、准确、灵敏, 能够适合用于小米中姜黄染色的定性、定量检查。

英文摘要:

      Objective To establish a method for detection of turmeric coloration in millet. Method Samples were extracted with acetic acid-methanol solution, then filtered, and then the obtained solution was evaporated and colored with boric acid-oxalic acid solution to prove the existence of turmeric. The filtered solution was detected by ultra performance liquid chromatography coupled with diode array detector (UPLC-DAD) for qualitative and quantitative analysis. Results The methodology experiments revealed that the coloration displayed sensitively. Turmeric had a good linear relationship in the concentration range of 1.0~20 μg/mL(equivalent to amount of 4~80 mg/kg), the value of precision (RSD) was less than 2%, and the average recovery was 99.8%. Conclusion The established method is rapid, accurate and sensitive, which is suitable for qualitative and quantitative detection of turmeric coloration in millet.